T-bet/GATA-3 ratio as a measure of the Th1/Th2 cytokine profile in mixed cell populations: predominant role of GATA-3
T-bet/GATA-3 ratio as a measure of the Th1/Th2 cytokine profile in mixed cell populations: predominant role of GATA-3
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DOI：
10.1016/s0022-1759(03)00200-x
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发表时间：
2003-07-01
影响因子：
2.2
通讯作者：
Scott, FW
中科院分区：
文献类型：
--
作者：
Chakir, H;Wang, HP;Scott, FW
初始T辅助细胞（Th）向Th 1或Th 2细胞的分化受T细胞中表达的转录因子T-box（T-bet）和GATA结合蛋白-3（加塔-3）调节。在本研究中，T-bet和加塔-3的基因表达的半定量逆转录聚合酶链反应（RT-PCR）测定的Th 1和Th 2细胞来源于纯化的脾CD 4（+）T细胞从DO11.10/Rag 2（-/-）转基因小鼠和对照生物育种（BBc）Wistar大鼠脾T细胞在Th 1或Th 2条件下刺激。在两组实验中，T-bet和加塔-3表达比例的变化反映了Th 1特异性细胞因子干扰素-γ（IFN-γ）和Th 2特异性细胞因子白细胞介素（IL）-4的变化。T-bet基因表达在完全极化的大鼠Th 1细胞中没有维持，而加塔-3基因表达在长期极化的大鼠Th 2细胞中维持，表明Th 1/Th 2状态的维持更多地是由于加塔-3 mRNA表达的改变而不是T-bet。这些转录因子在产生1型和2型细胞因子的几种细胞中被上调，并且可以使用从混合细胞群或培养的脾细胞分离的总RNA通过RT-PCR容易地分析，从而提供在各种条件下Th 1/Th 2细胞因子平衡的替代标记。(C)2003 Elsevier B. V.保留所有权利。
The differentiation of naive T-helper (Th) cells towards Th1 or Th2 cells is regulated by the transcription factors T-box expressed in T-cells (T-bet) and GATA-binding protein-3 (GATA-3). In the present study, the gene expression of T-bet and GATA-3 was measured by semi-quantitive reverse transcription polymerase chain reaction (RT-PCR) in Th1 and Th2 cells derived from purified splenic CD4(+) T cells from DO11.10/Rag2(-/-) transgenic mice and control BioBreeding (BBc) Wistar rat splenic T cells stimulated under Th1 or Th2 conditions. In both sets of experiments, changes in the ratio of expression of T-bet and GATA-3 reflected changes in the Th1-specific cytokine interferon-gamma (IFN-gamma) and Th2-specific cytokine interleukin (IL)-4. T-bet gene expression was not maintained in fully polarized rat Th1 cells whereas GATA-3 gene expression was maintained in long-term polarized rat Th2 cells, indicating that maintenance of Th1/Th2 status occurred more as a result of altered GATA-3 mRNA expression than T-bet. These transcription factors are up-regulated in several cells that produce type 1 and type 2 cytokines and can be analyzed readily by RT-PCR using total RNA isolated from mixed cell populations or cultured splenocytes thereby providing a surrogate marker of Th1/Th2 cytokine balance under a variety of conditions. (C) 2003 Elsevier B.V. All rights reserved.