cDNA cloning, expression pattern, genomic structure and chromosomal location of RAB6KIFL, a human kinesin-like gene
cDNA cloning, expression pattern, genomic structure and chromosomal location of RAB6KIFL, a human kinesin-like gene
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DOI：
10.1016/s0378-1119(00)00135-9
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发表时间：
2000-05-02
期刊：
影响因子：
3.5
通讯作者：
Le Beau, MM
中科院分区：
文献类型：
--
作者：
Lai, F;Fernald, AA;Le Beau, MM
驱动蛋白样蛋白是微管相关的马达，在细胞内运输和细胞分裂中发挥重要作用。我们在这里报告了一种新的人类驱动蛋白样蛋白 Rabkinesin6 (RAB6KIFL) 的表征。复合cDNA序列为2957 bp，编码890个氨基酸的蛋白质，预计分子量为100 kDa。它与小鼠驱动蛋白样蛋白Rab6kifl具有高度同源性（93%相似性），表明它是小鼠基因的人类同源物。 RAB6KIFL 具有微管相关马达功能所需的所有结构特征。与普遍表达的小鼠基因不同，RAB6KIFL 表达似乎具有组织特异性。它在胎儿组织中广泛表达，在成人胸腺、骨髓和睾丸中大量表达，在心脏、胎盘和脾脏中低水平表达。 RAB6KIFL 基因定位于人类 5 号染色体 q31 条带，跨度类似于 8.5 kb 的基因组 DNA，包含 19 个外显子。 RAB6KIFL 位于以 5q 缺失为特征的髓系白血病中最小的常见缺失片段内；然而，我们在 5q 缺失的恶性骨髓疾病中未检测到 RAB6KIFL 突变。这种人类驱动蛋白样蛋白的描述可以帮助人们更好地理解这一大蛋白家族的多样性。 (C) 2000 年由 Elsevier Science B.V. 出版。保留所有权利。
Kinesin-like proteins are microtubule-associated motors that play important roles in intracellular transport and cell division. We report here the characterization of a new human kinesin-like protein, Rabkinesin6 (RAB6KIFL). The composite cDNA sequence is 2957 bp, and encodes a protein of 890 amino acids with a predicted molecular weight of 100 kDa. It has high homology (93% similarity) with the mouse kinesin-like protein, Rab6kifl, indicating that it is the human homologue of the mouse gene. RAB6KIFL has all of the structural characteristics required to function as a microtubule-associated motor. Unlike the mouse gene which is ubiquitously expressed, RAB6KIFL expression appears to be tissue specific. It is widely expressed in fetal tissues, abundantly expressed in adult thymus, bone marrow and testis, and is expressed at low levels in heart, placenta and spleen. The RAB6KIFL gene is mapped to human chromosome 5, band q31, spans similar to 8.5 kb of genomic DNA, and contains 19 exons. RAB6KIFL maps within the smallest commonly deleted segment in myeloid leukemias characterized by a deletion of 5q; however, we detected no mutations of RAB6KIFL in malignant myeloid disorders with loss of 5q. The description of this human kinesin-like protein may provide a better understanding of the diversity of this large family of proteins. (C) 2000 Published by Elsevier Science B.V. All rights reserved.