Mid-trimester fetal livers are a rich source of CD34+/++ cells for transplantation
Mid-trimester fetal livers are a rich source of CD34+/++ cells for transplantation
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DOI：
10.1038/sj.bmt.1701940
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发表时间：
1999-09-01
影响因子：
4.8
通讯作者：
Muench, MO
中科院分区：
文献类型：
--
作者：
Golfier, F;Bárcena, A;Muench, MO
研究了妊娠16周至24周的人胎肝(FL)作为产前和出生后移植的造血干细胞来源的潜力。在这份报告中，我们对人类FL作为候选干细胞的来源进行了定量评估，并制定了一种分离这些细胞的方案，这些细胞没有微生物污染，几乎没有成熟的T细胞。人FLS中位数为1.9×10(9)个活细胞，平均为1.3×10(8)个CD34(+/++)细胞(范围1.1×10(7)至4.7×10(8))。无论胎龄，造血祖细胞总数或候选干细胞数量(CD34(++)CD38(-)和CD34(++)CD(4+))均无显著差异，提示造血祖细胞在肝脏中的扩张在孕16周后达到平台期。集落形成单位培养(CFU-C)为4.1×10(6)~2.5×10(7)个/FL。用Baxter Isolex 50细胞分选仪对FL CD34(++)细胞进行阳性选择。CD34(+/++)细胞的平均纯度为74%，得率为29%。T细胞被耗尽了99.95%，导致平均每个处理的肝脏有6.5×10(3)T细胞。对候选干细胞群体和原始集落形成细胞(CFCs)的分析表明，这些细胞比CD34(+/++)细胞群体更具优势。经处理的CD34(+/++)细胞被发现是无菌的。总之，在妊娠16-24周对FL祖细胞进行纯化可以产生大量的早期祖细胞，适合于宫内移植和可能的出生后移植。
Human fetal livers (FL), between 16 and 24 weeks of gestation, were studied for their potential as a source of hematopoietic stem cells for prenatal and postnatal transplantation. In this report we give a quantitative evaluation of human FL as a source of candidate stem cells, and develop a protocol for the isolation of these cells free of microbial contaminants and almost free of mature T cells. Human FLs contained a median 1.9 x 10(9) viable cells and a mean of 1.3 x 10(8) CD34(+/++) cells (range 1.1 x 10(7) to 4.7 x 10(8)). Regardless of gestational age, no significant differences mere apparent in the numbers of total progenitors or in the numbers of candidate stem cells (CD34(++) CD38(-) and CD34(++)CD(4+)), suggesting that the expansion in the liver of the early compartments of hematopoietic progenitors reaches a plateau after the sixteenth week of gestation. Colony-forming units culture (CFU-C) were found to range from 4.1 x 10(6) to 2.5 x 10(7) per FL. Positive selection of FL CD34(++) cells was achieved using the Baxter Isolex 50 device. An average purity of 74% and yield of 29% of CD34(+/++) cells was achieved. T cells were depleted by 99.95%, resulting in a mean of 6.5 x 10(3) T cells per processed liver. Analysis of candidate stem cell populations and primitive colony-forming cells (CFC) suggested a preferential enrichment of these cells over the total population of CD34(+/++) cells. Processed CD34(+/++) cells were found to be sterile. In conclusion, purification of FL progenitors between 16 and 24 weeks of gestation results in a large number of early progenitors suitable for irt utero and possibly postnatal transplantation.