Intramolecular DNA coiling mediated by metallosupramolecular cylinders: Differential binding of P and M helical enantiomers
Intramolecular DNA coiling mediated by metallosupramolecular cylinders: Differential binding of P and M helical enantiomers
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DOI：
10.1073/pnas.062634499
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发表时间：
2002-04-16
影响因子：
11.1
通讯作者：
Hannon, MJ
中科院分区：
文献类型：
--
作者：
Meistermann, I;Moreno, V;Hannon, MJ
我们设计了一种合成的四价阳离子金属-超分子圆柱体，它靶向DNA的大沟，结合常数超过10（7）M-1，并诱导DNA弯曲和分子内卷曲。螺旋分子的两种对映体与DNA的结合方式不同，具有不同的结构效应。我们报告的表征的相互作用的一系列生物物理技术。M螺旋圆柱体与大沟结合并诱导显著的分子内卷曲。对于P对映体来说，DNA弯曲不太明显。
We have designed a synthetic tetracationic metallo-supramolecular cylinder that targets the major groove of DNA with a binding constant in excess of 10(7) M-1 and induces DNA bending and intramolecular coiling. The two enantiomers of the helical molecule bind differently to DNA and have different structural effects. We report the characterization of the interactions by a range of biophysical techniques. The M helical cylinder binds to the major groove and induces dramatic intramolecular coiling. The DNA bending is less dramatic for the P enantiomer.