Soybean nuclear factor YA10 positively regulates drought resistance in transgenic Arabidopsis thaliana
Soybean nuclear factor YA10 positively regulates drought resistance in transgenic Arabidopsis thaliana
复制标题
大豆核因子YA10正向调节转基因拟南芥的抗旱性
DOI：
10.1016/j.envexpbot.2020.104249
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发表时间：
2020-09
影响因子：
5.7
通讯作者：
Yuehua Yu;Yucui Bai;Ping Wang;Yi Wang;Huina Wan;Chen Liu;Z. Ni
中科院分区：
文献类型：
--
作者：
Yuehua Yu;Yucui Bai;Ping Wang;Yi Wang;Huina Wan;Chen Liu;Z. Ni
Nuclear factor (NF)-Y is composed of three subunits, NF-YA, NF-YB and NF-YC, and plays important roles in the regulation of plant abiotic stress response gene expression. However, the function of individual soybean NF-YA proteins in response to drought stress is unclear. In this study, the soybean NF-YA subunit geneGmNF-YA10was cloned, and its function in response to drought stress was identified.GmNF-YA10is broadly expressed in soybean tissues and is specifically located in the nucleus. In soybean roots, the transcription level of theGmNF-YA10gene was induced by polyethylene glycol (PEG)-simulated drought stress and abscisic acid (ABA) treatment. Under mannitol stress, the germination rate of transgenicArabidopsisoverexpressingGmNF-YA10was higher than that of wild-type (WT)Arabidopsis. After drought stress, the survival rate, relative water content, ABA content, proline content and chlorophyll content of transgenicArabidopsisoverexpressingGmNF-YA10were higher than those of WTArabidopsiswhile the rate of water loss and the contents of malondialdehyde and sucrose were lower than those of WTArabidopsis, indicating that transgenicArabidopsisoverexpressingGmNF-YA10has better drought resistance than WTArabidopsis. Under the ABA treatment, the root length of transgenicArabidopsisoverexpressingGmNF-YA10was shorter than that of WTArabidopsisand the degree of stomatal closure was greater than that of WTArabidopsis, indicating that transgenicArabidopsisoverexpressingGmNF-YA10was more sensitive to ABA at the post-germination stage. Transcriptome sequencing and real-time quantitative PCR analysis showed thatGmNF-YA10affected the expression of both ABA-dependent and ABA-independent signalling pathway genes. These results indicate that GmNF-YA10 significantly improves the drought resistance of transgenicArabidopsisthrough ABA-dependent and ABA-independent signal transduction pathways.
