Conditional gene-trap mutagenesis in zebrafish.
Conditional gene-trap mutagenesis in zebrafish.
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DOI：
10.1007/978-1-62703-721-1_19
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
Chen, Wenbiao
中科院分区：
文献类型：
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作者：
Maddison, Lisette A;Li, Mingyu;Chen, Wenbiao
斑马鱼已成为基因功能分析中广泛使用的模型。已经使用了几种方法来在这种生物体中产生突变，并且可以获得数千种突变株系。然而，所有常规的斑马鱼突变都会影响所有细胞中的基因，这使得很难确定组织特异性功能。我们采用FlEx Trap方法通过基因陷阱诱变在斑马鱼中产生条件突变。与合适的Cre或Flp系组合，插入突变体不仅允许空间和时间特异性基因失活，而且允许空间和时间特异性拯救被破坏的基因。我们提供了如何产生和使用这种突变的实验细节。
Zebrafish has become a widely used model for analysis of gene function. Several methods have been used to create mutations in this organism and thousands of mutant lines are available. However, all the conventional zebrafish mutations affect the gene in all cells at all time, making it difficult to determine tissue-specific functions. We have adopted a FlEx Trap approach to generate conditional mutations in zebrafish by gene trap mutagenesis. Combined with appropriate Cre or Flp lines, the insertional mutants not only allow spatial and temporal specific gene inactivation, but also permit spatial and temporal specific rescue of the disrupted gene. We provide experimental details on how to generate and use such mutations.