ParaHydrogen Induced Polarization of 13C carboxylate resonance in acetate and pyruvate
ParaHydrogen Induced Polarization of 13C carboxylate resonance in acetate and pyruvate
复制标题
DOI：
10.1038/ncomms6858
复制
发表时间：
2015-01-01
影响因子：
16.6
通讯作者：
Aime, Silvio
中科院分区：
文献类型：
--
作者：
Reineri, Francesca;Boi, Tommaso;Aime, Silvio
核自旋超极化技术的出现代表了磁共振成像医学诊断领域的突破。动态核极化 (DNP) 是最广泛使用的方法，超极化代谢物如 [1-C-13]-丙酮酸可报告肿瘤的状态。仲氢诱导极化 (PHIP) 是一种基于化学的技术，与 DNP 相比，更易于处理且成本更低，并且极化时间显着缩短。其主要限制是目标基材不饱和前体的可用性；例如，乙酸盐和丙酮酸盐不能通过直接掺入仲氢分子来获得。在此，我们报告了一种方法，该方法允许我们通过含有可氢化官能团的定制前体实现此类分子的超极化，该前体在极化转移到目标 C-13 部分后被裂解以获得感兴趣的代谢物。所报告的程序可以扩展到许多其他生物学相关的底物。
The advent of nuclear spins hyperpolarization techniques represents a breakthrough in the field of medical diagnoses by magnetic resonance imaging. Dynamic nuclear polarization (DNP) is the most widely used method, and hyperpolarized metabolites such as [1-C-13]-pyruvate are shown to report on status of tumours. Parahydrogen-induced polarization (PHIP) is a chemistry-based technique, easier to handle and much less expensive in respect to DNP, with significantly shorter polarization times. Its main limitation is the availability of unsaturated precursors for the target substrates; for instance, acetate and pyruvate cannot be obtained by direct incorporation of the parahydrogen molecule. Herein we report a method that allows us to achieve hyperpolarization in this kind of molecule by means of a tailored precursor containing a hydrogenable functionality that, after polarization transfer to the target C-13 moiety, is cleaved to obtain the metabolite of interest. The reported procedure can be extended to a number of other biologically relevant substrates.