Antioxidative pyranonigrins in rice mold starters and their suppressive effect on the expression of blood adhesion molecules
Antioxidative pyranonigrins in rice mold starters and their suppressive effect on the expression of blood adhesion molecules
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DOI：
10.1271/bbb.80077
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发表时间：
2008-06-01
影响因子：
1.6
通讯作者：
Osawa, Toshihiko
中科院分区：
文献类型：
--
作者：
Miyake, Yoshiaki;Mochizuki, Mika;Osawa, Toshihiko
对用于制备各种日本发酵食品的米霉菌发酵剂中具有1，1-二苯基-2-苦基肼（DPPH）自由基清除能力的抗氧化剂及其对血液粘附分子表达的有效性进行了检测。从烧酒用米霉发酵剂中分离出一种抗氧化剂，经H-1-NMR、C-13-NMR和FAB-MS分析鉴定为吡喃黑色素-S（PG-S）。它是吡喃黑素-A（PG-A）的衍生物，其已作为抗氧化剂从米霉菌发酵剂中分离出来。用泡盛曲霉、A. kawachii和A.斋藤PG-S对DPPH自由基的清除活性高于PG-A。PG-A对肿瘤坏死因子-α（TNF-α）诱导的人脐静脉内皮细胞（HUVECs）表达血管细胞粘附分子-1（VCAM-1）有明显的抑制作用（P < 0.05）。
Antioxidants having a 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging capacity in rice mold starters, which are used for the preparation of various Japanese fermented foods, and their effectiveness against the expression of blood adhesion molecules were examined. An antioxidant was isolated from the rice mold starters used for shochu and identified as pyranonigrin-S (PG-S) by H-1-NMR, C-13-NMR, and FAB-MS analyses. It was a derivative of pyranonigrin-A (PG-A), which has been isolated as an antioxidant from the rice mold starters. Pyranonigrins PG-A and PG-S were found to exist in spores on rice mold starters which had been prepared by Aspergillus awamori, A. kawachii, and A. saitoi. PG-S exhibited a higher level of DPPH radical scavenging activity than PG-A. PG-A was found to have a significant suppressive effect on the expression of vascular cell adhesion molecule-1 (VCAM-1) in human umbilical vein endothelial cells (HUVECs) induced by tumor necrosis factor-a (TNF-alpha) (P < 0.05).