Co-fermentation of a mixture of glucose and xylose to hydrogen by Thermoanaerobacter thermosaccharolyticum W16: Characteristics and kinetics
Co-fermentation of a mixture of glucose and xylose to hydrogen by Thermoanaerobacter thermosaccharolyticum W16: Characteristics and kinetics
复制标题
热解糖热厌氧杆菌 W16 将葡萄糖和木糖混合物共发酵制氢：特性和动力学
DOI：
10.1016/j.ijhydene.2019.02.125
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发表时间：
2019
影响因子：
7.2
通讯作者：
Nanqi Ren
中科院分区：
文献类型：
--
作者：
Lei Zhao;Zi-Han Wang;Jie-Ting Wu;Hong-Yu Ren;Shan-Shan Yang;Jun Nan;Guang-Li Cao;Ya-Chun Sheng;Aijie Wang;Nanqi Ren
摘要葡萄糖和木糖共发酵是最大限度地利用废弃木质纤维素产氢的关键。在这项研究中，细胞的生长，糖消耗，和产氢曲线的ThermoanaerobiumthermosaccharolyticumW 16饲料与一系列的葡萄糖和木糖的实验研究，再加上动力学分析。结果表明，虽然T. Thermosaccharolyticum W16能同时利用葡萄糖和木糖产氢，以葡萄糖为唯一碳源时，最大细胞生长速率为0.27g/L/h，产氢速率为14.53mmol/L/h，分别比以木糖为唯一碳源时高92.8%和49.8%。进一步的插值分析和实验论证表明，当混合底物中葡萄糖含量高于58.2%时，对木糖利用的抑制作用增强，而当葡萄糖含量低于21.7%时，其利用会受到一定程度的反馈抑制。实验结果与动力学分析相结合，为进一步开发T.热解糖菌（thermosaccharolyticum）W16作为用于从木质纤维素生物质制氢的生物催化剂。
Abstract Glucose and xylose co-fermentation is crucial to maximize hydrogen yield from waste lignocellulose. In this study, cell growth, sugar consumption, and hydrogen production profiles of Thermoanaerobacter thermosaccharolyticum W16 feeding with a range of glucose and xylose were experimental investigated coupled with kinetic analysis. Results showed although T. thermosaccharolyticum W16 could use both glucose and xylose for hydrogen production, a maximum cell growth rate of 0.27g/L/h and hydrogen production rate of 14.53mmol/L/h was found with glucose as sole substrate, the value was 92.8% and 49.8% higher than using xylose as the only carbon source. Further interpolation analysis and experimental demonstration suggested when glucose content in the mixed substrate higher than 58.2%, the inhibitory effect on xylose utilization was increased, but when glucose concentration fell below 21.7%, its utilization will be subject to a certain degree of feedback inhibition. Coupling experimental results with kinetic analysis in this study provides a powerful evidence to further develop the potential of T. thermosaccharolyticum W16 as a biocatalyst for hydrogen production from lignocellulosic biomass.