CO2 dialysis in nucleus tractus solitarius region of rat increases ventilation in sleep and wakefulness
CO2 dialysis in nucleus tractus solitarius region of rat increases ventilation in sleep and wakefulness
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DOI：
10.1152/japplphysiol.01128.2001
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发表时间：
2002-05-01
影响因子：
3.3
通讯作者：
Li, AH
中科院分区：
文献类型：
--
作者：
Nattie, EE;Li, AH
为了评估大鼠在睡眠和清醒时广泛分布的中枢化学感受器的功能，我们在自然发生的睡眠-觉醒周期中，通过微透析25%二氧化碳平衡的人工脑脊液来局部刺激单个化学感受器位点。在后锥体核中，仅在清醒状态下，这种方法增加了24%的通气量（潮气量）（Li A, Randall M, and Nattie E. J .应用生理学杂志，87:910-919,1999）。在尾侧延髓缝中，它仅在睡眠时增加通气（频率）15-20% （Nattie EE和Li A. J . journal of clinical physiology, 90: 1247-1257, 2001）。在孤立束核（NTS）中，局灶酸化显著增加了睡眠时11%和清醒时7%的通气量（n = 5），睡眠时16%和清醒时28%的通气量（n = 5）。睡眠-觉醒周期没有改变。5% CO2透析无效果。二氧化碳浓度为50%的透析不能进一步刺激通气，但会干扰睡眠。NTS的中枢化学感受器影响睡眠和清醒时的呼吸。尾侧NTS的觉醒阈值高于呼吸刺激的阈值。
To evaluate the function of widely distributed central chemoreceptors during sleep and wakefulness in the rat, we focally stimulate single chemoreceptor sites during naturally occurring sleep-wake cycles by microdialysis of artificial cerebrospinal fluid equilibrated with 25% CO2. In retrotrapezoid nucleus, this increased ventilation (tidal volume) by 24% only in wakefulness (Li A, Randall M, and Nattie E. J Appl Physiol 87: 910-919, 1999). In caudal medullary raphe, it increased ventilation (frequency) by 15-20% only in sleep (Nattie EE and Li A. J Appl Physiol 90: 1247-1257, 2001). Here, in nucleus tractus solitarius (NTS), focal acidification significantly increased ventilation by 11% in sleep and 7% in wakefulness rostrally (n = 5) and by 16% in sleep and 28% in wakefulness caudally (n = 5). The sleep-wake cycle was unaltered. Dialysis with 5% CO2 had no effect. Dialysis with 50% CO2 caudally did not further stimulate ventilation but did disrupt sleep. Central chemoreceptors in the NTS affect breathing in both sleep and wakefulness The threshold for arousal in caudal NTS is greater than that for the stimulation of breathing.