Acute effects of antisense antagonism of a single peptide neurotransmitter in the circadian clock.
Acute effects of antisense antagonism of a single peptide neurotransmitter in the circadian clock.
复制标题
单肽神经递质反义拮抗作用对生物钟的急性影响。
DOI：
10.1152/ajpregu.1996.270.1.r283
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Wise,PM
中科院分区：
文献类型：
--
作者：
Scarbrough,K;Harney,JP;Rosewell,KL;Wise,PM
位于下丘脑视交叉上核 (SCN) 的生物钟是驱动哺乳动物大多数 24 小时节律的主要神经起搏器。几种神经递质肽在该细胞核内合成，并与 SCN 中的其他细胞以及大脑其他区域的细胞有节奏地通讯。目前，人们对它们在调节时钟输出方面的作用知之甚少。我们证明，将对应于 NH2 末端和血管活性肠肽 (VIP) mRNA 翻译起始位点的反义寡脱氧核苷酸注入大鼠视交叉上区域，可暂时消除皮质酮分泌的昼夜节律，而不影响同一动物中与应激相关的皮质酮分泌。反义治疗后第二天，VIP 肽水平被抑制 30%。这些结果表明，生物钟中的单个神经肽递质可能在控制特定昼夜节律中发挥独特的作用。
The circadian clock that resides in the suprachiasmatic nucleus (SCN) of the hypothalamus is the major neural pacemaker driving most 24-h rhythms in mammals. Several neurotransmitter peptides are synthesized within this nucleus and communicate rhythmically with other cells in the SCN as well as with cells in other regions of the brain. At the present time, little is known about their role in regulating outputs of the clock. We demonstrate that antisense oligodeoxynucleotides corresponding to the NH2-terminus and the translation start site of vasoactive intestinal peptide (VIP) mRNA infused into the suprachiasmatic region of rats temporarily abolishes the circadian rhythm of corticosterone secretion without influencing stress-related corticosterone secretion in the same animals. Levels of VIP peptide are suppressed 30% on the second day after antisense treatment. These results indicate that a single neuropeptide transmitter in the circadian clock may serve a distinct role in the control of specific circadian rhythms.