Physicochemical characterization, antioxidant activity of polysaccharides from Mesona chinensis Benth and their protective effect on injured NCTC-1469 cells induced by H2O2
Physicochemical characterization, antioxidant activity of polysaccharides from Mesona chinensis Benth and their protective effect on injured NCTC-1469 cells induced by H2O2
复制标题
凉草多糖的理化性质、抗氧化活性及其对H2O2诱导的NCTC-1469细胞损伤的保护作用
DOI：
10.1016/j.carbpol.2017.08.018
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发表时间：
2017-11-01
影响因子：
11.2
通讯作者：
Xie, Mingyong
中科院分区：
文献类型：
--
作者：
Tang, Wei;Shen, Mingyue;Xie, Mingyong
Polysaccharides MP-A, MP-U and MP-C were extracted from Mesona chinensis Benth by hot-alkali extraction (HAE), ultrasonic-assisted extraction (UAE) and cellulose-enzyme assisted extraction (CAE), respectively. The yields, physicochemical properties and antioxidant of polysaccharides were investigated. Results showed that the yields of MP-A (11.14%), MP-U (10.62%) and MP-C (9.70%) were similar, and they were all heteropolysaccharides with average molecular weights of 1.9 x 10(5) Da, 1.5 x 10(5) Da, 1.4 x 10(5) Da. Glucose, galactose, and galacturonic acid were the main monosaccharides in MP-A, MPU and MP-C with molar ratios of 1.00:1.34:0.25, 1.00:2.49:0.19 and 1.00:2.95:0.84, respectively. MP-C extracted by CAE exhibited higher antioxidant activities in FRAP, DPPH, hydroxyl radical assays and the H2O2-induced injury cell model. The three extraction methods had only slight effects on chemical composition, while MP-C extracted by CAE exhibited the highest antioxidant activity, which could potentially be used for an addition ingredient as it was previously shown to have good gelling property in food products. (C) 2017 Elsevier Ltd. All rights reserved.
