Direct Evidence for the Up-regulation of Vps34 Regulated by PKCγ During Short-Term Treatment With Morphine
Direct Evidence for the Up-regulation of Vps34 Regulated by PKCγ During Short-Term Treatment With Morphine
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DOI：
10.1002/syn.20612
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发表时间：
2009-05-01
期刊：
影响因子：
2.3
通讯作者：
Ohkuma, Seitaro
中科院分区：
文献类型：
--
作者：
Shebasaki, Masahiro;Kurokawa, Kazuhiro;Ohkuma, Seitaro
在这项研究中，我们调查是否PKC γ可以与空泡蛋白分选34（Vps 34）的功能变化，在吗啡治疗过程中，使用原代培养的小鼠大脑皮层神经元。免疫沉淀分析表明p-PKC γ和Vps 34共同存在于分子复合物中。吗啡治疗增加PKC γ和Vps 34水平。PKC γ磷酸化增加Vps 34水平。抑制吗啡诱导的PKC γ磷酸化的增加降低了Vps 34水平。这些结果表明，阿片受体激活增加PKC γ磷酸化的神经元，反过来，上调Vps 34在短期治疗神经元。Synapse 63：365-368，2009. (C)2009 Wiley-Liss，Inc.
In this study, we investigated whether PKC gamma could be associated with functional changes of vacuolar protein sorting 34 (Vps34) during morphine treatment using primary cultures of cerebral cortical neurons from mice. The immunoprecipitation analysis showed that p-PKC gamma and Vps34 are present together in molecular complexes. The treatment with morphine increases PKC gamma and Vps34 levels. Phosphorylation of PKC gamma increased Vps34 level. The inhibition of morphine-induced increase in PKC gamma phosphorylation reduced Vps34 level. These results indicates that opioid receptor activation increases PKC gamma phosphorylation in the neurons and, in turn, upregulates Vps34 during short-term treatment with neurons. Synapse 63:365-368, 2009. (C) 2009 Wiley-Liss, Inc.