Long non-coding RNA CARLo-5 is a negative prognostic factor and exhibits tumor pro-oncogenic activity in non-small cell lung cancer
Long non-coding RNA CARLo-5 is a negative prognostic factor and exhibits tumor pro-oncogenic activity in non-small cell lung cancer
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DOI：
10.1007/s13277-014-2442-7
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发表时间：
2014-11-01
期刊：
影响因子：
--
通讯作者：
Zheng, Di
中科院分区：
文献类型：
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作者：
Luo, Jie;Tang, Liang;Zheng, Di
尽管近年来非小细胞肺癌（NSCLC）的治疗取得了进展，但NSCLC的预后仍然相对较差。因此，寻找新的有效的NSCLC诊断和预后生物标志物具有重要意义。越来越多的文献已经证明了长链非编码RNA（lncRNA）在癌症生物学中的重要作用。癌症相关区长非编码RNA（CARLo-5），最近发现的lncRNA，最初的特点是在结肠癌。然而，关于它在NSCLC中的作用知之甚少。在本研究中，与其相邻的正常组织相比，在癌组织中观察到CARLo-5的大幅上调。同时，CARLo-5高表达患者的预后明显差于低表达患者。通过siRNA抑制CARLo-5在体外抑制NSCLC细胞系中的增殖、迁移和侵袭。此外，CARLo-5的沉默逆转了NSCLC细胞系中的上皮-间充质转化。我们的研究表明，CARLo-5可能代表NSCLC的预后标志物和潜在的治疗靶点。
Despite recent advances in the management of non-small cell lung cancer (NSCLC), the prognosis of NSCLC is still relatively poor. Thus, it is of great importance to identify novel effective diagnostic and prognostic biomarker of NSCLC. A growing volume of literature has demonstrated the vital roles of long non-coding RNAs (lncRNAs) in cancer biology. Cancer-associated region long non-coding RNA (CARLo-5), a recently identified lncRNA, was initially characterized in colon cancer. However, little is known about its role in NSCLC. In the present study, a great upregulation of CARLo-5 was observed in cancer tissues compared to their adjacent normal tissues. Meanwhile, patients with high CARLo-5 expression have significantly poorer prognosis than those with low expression. Inhibition of CARLo-5 by siRNA suppressed the proliferation, migration, and invasion in NSCLC cell lines in vitro. In addition, silencing of CARLo-5 reversed the epithelial-mesenchymal transition in NSCLC cell line. Our study suggests that CARLo-5 may represent a prognostic marker and a potential therapeutic target of NSCLC.