A divergent CFTR homologue: highly regulated salt transport in the euryhaline teleost F. heteroclitus.
A divergent CFTR homologue: highly regulated salt transport in the euryhaline teleost F. heteroclitus.
复制标题
不同的CFTR同源物：广盐性硬骨鱼F.heteroclitus中高度调控的盐运输。
DOI：
10.1152/ajpcell.1998.274.3.c715
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
C. Higgins
中科院分区：
文献类型：
--
作者：
T. D. Singer;S. Tucker;W. Marshall;C. Higgins
鳉是广盐性硬骨鱼，能迅速适应从淡水到四倍海水的迁移。为了在分子水平上研究胞浆蛋白酶的调控，从适应SW的鳉鱼的鳃cDNA文库中分离了与人囊性纤维化跨膜传导调节因子（hCFTR）同源的5.7-胞浆蛋白酶cDNA。该cDNA编码的蛋白质产物（kfCFTR）与hCFTR（迄今为止表征的CFTR的最不同形式）具有59%的同一性。kfCFTR在非洲爪蟾卵母细胞中的表达产生了与hCFTR类似的腺苷3 '，5'-环磷酸激活的Cl-选择性电流。在SW适应的鳉鱼，kfCFTR表达在高水平的鳃，鳃盖上皮，和肠。突然暴露后的FW适应的鳉鱼SW，kfCFTR在鳃中的表达增加了几倍，这表明kfCFTR在盐度适应的作用。在类似条件下，血浆Na+水平在8 h后显著升高，然后下降，尽管尚不清楚这些变化是否直接导致kfCFTR表达的变化。该鳉鱼提供了一个独特的机会，了解硬骨鱼的调节和CFTR的作用。
The killifish, Fundulus heteroclitus, is a euryhaline teleost fish capable of adapting rapidly to transfer from freshwater (FW) to four times seawater (SW). To investigate osmoregulation at a molecular level, a 5.7-kilobase cDNA homologous to human cystic fibrosis transmembrane conductance regulator (hCFTR) was isolated from a gill cDNA library from SW-adapted killifish. This cDNA encodes a protein product (kfCFTR) that is 59% identical to hCFTR, the most divergent form of CFTR characterized to date. Expression of kfCFTR in Xenopus oocytes generated adenosine 3',5'-cyclic monophosphate-activated, Cl--selective currents similar to those generated by hCFTR. In SW-adapted killifish, kfCFTR was expressed at high levels in the gill, opercular epithelium, and intestine. After abrupt exposure of FW-adapted killifish to SW, kfCFTR expression in the gill increased severalfold, suggesting a role for kfCFTR in salinity adaptation. Under similar conditions, plasma Na+levels rose significantly after 8 h and then fell, although it is not known whether these changes are directly responsible for the changes in kfCFTR expression. The killifish provides a unique opportunity to understand teleost osmoregulation and the role of CFTR.
没有外显子 5 的人上皮囊性纤维化跨膜电导调节器维持细胞内膜中的部分氯离子通道功能。
DOI：
10.1016/s0006-3495(96)79508-5
发表时间：
1996
期刊：
Biophysical journal.
影响因子：
--
作者：
Xie,J;Drumm,ML;Zhao,J;Ma,J;Davis,PB
通讯作者：
Davis,PB
影响因子：
56.9
作者：
FOSKETT, JK;SCHEFFEY, C
通讯作者：
SCHEFFEY, C