Asymmetric morphology of the cells comprising the inner and outer bending sides of the murine duodenojejunal flexure
Asymmetric morphology of the cells comprising the inner and outer bending sides of the murine duodenojejunal flexure
复制标题
鼠十二指肠空肠弯曲内、外弯曲侧细胞的不对称形态
DOI：
10.1007/s00441-014-2091-6
复制
发表时间：
2015
影响因子：
3.6
通讯作者：
Yasuhiro Kon
中科院分区：
文献类型：
--
作者：
Sawa Onouchi;Osamu Ichii;Saori Otsuka-Kanazawa;Yasuhiro Kon
组成细胞的不对称形状决定了发育器官的不对称特征。在这里，我们以小鼠十二指肠空肠弯曲(DJF)为研究对象，分析了胚胎10.75-11.75天内、外弯曲侧粘膜上皮和肠壁细胞的形态不对称性。粘膜上皮细胞形态及上皮标志物(CDX2、E-cadherin)的表达在双侧无明显差异。DJF内侧和外侧的肠壁细胞分别沿内外轴线和垂直于内外轴方向拉长。此外，外侧的肠壁细胞具有通过贴壁连接连接细胞的细胞质突起，而内侧的肠壁细胞通过并列细胞体的贴壁连接连接，相对较拥挤。免疫组织化学染色显示，间充质细胞(Vimentin)、平滑肌细胞(α-SMA)、内皮细胞(LYVE-1、CD34)、未分化神经元(SOX10)的阳性反应在两组间差异无统计学意义。有趣的是，表明分化神经元的Tuj1阳性细胞出现在肠壁的中层，这些细胞明显更丰富，并且倾向于内侧比外侧大。总之，我们阐明了DJF内侧和外侧肠壁细胞形态和神经分化的不对称性。发育中的小鼠DJF的这些特征表明它的不对称形成。
The asymmetric shape of component cells determines the asymmetric features of developing organs. Here, we focused on the murine duodenojejunal flexure (DJF), which bends without affecting the mesentery, and analyzed the morphological asymmetries of the mucosal epithelium and gut wall cells between the inner and outer bending sides at embryonic days 10.75–11.75. In the mucosal epithelium, the cell shape and the expression of epithelial markers (Cdx2, E-cadherin) showed no differences between the two DJF sides. In contrast, the gut wall cells comprising the inner and outer sides of the DJF were elongated along the inner–outer axis and perpendicular to this axis, respectively. Furthermore, the gut wall cells in the outer side possessed cytoplasmic processes connecting cells via adherens junctions, but those in the inner side were attached via adherens junctions of juxtaposed cell bodies and were relatively more crowded. In immunohistochemistry experiments, there was no remarkable difference in the positive reactions of markers for mesenchyme (vimentin), smooth muscle cells (αSMA), endothelial cells (LYVE-1, CD34), and undifferentiated neurons (Sox10) between the DJF sides. Interestingly, Tuj1-positive cells, indicating differentiated neurons, were observed in the middle layer of the gut wall, and these cells were significantly more abundant and tended to be larger in the inner side than in the outer side of the DJF. In conclusion, we clarified the asymmetries of gut wall cell morphology and neural differentiation between the inner and outer sides of the DJF. These characteristics of the developing murine DJF indicate its asymmetric formation.