Phenotypic Heterogeneity of Pseudomonas aeruginosa Populations in a Cystic Fibrosis Patient
Phenotypic Heterogeneity of Pseudomonas aeruginosa Populations in a Cystic Fibrosis Patient
复制标题
囊性纤维化患者中铜绿假单胞菌种群的表型异质性
作者：
M. Workentine;C. Sibley;B. Glezerson;Swathi Purighalla;Jens C. Norgaard;M. Parkins;H. Rabin;M. Surette
条件致病菌铜绿假单胞菌慢性感染囊性纤维化患者的下呼吸道。在整个感染过程中，这种微生物经历了有助于其在呼吸道中长期存在的适应。虽然已经记录了铜绿假单胞菌的多样性，但不太清楚患者内部的多样性对总体种群结构的影响程度，因为大多数研究仅限于分析每个患者每个时间点的少数几个分离株。为了更详细地检查铜绿假单胞菌的种群结构，我们从一名长期定植于铜绿假单胞菌的患者的个人痰样本中收集了多个分离株。该菌株收集包括169个克隆性分离株，代表了三个肺恶化和临床稳定期，分析了广泛的表型选择。这些表型包括菌落形态、运动性、群体感应、蛋白酶活性、营养缺陷性、铁载体水平、抗生素耐药性和生长曲线。即使在同一样本的同一菌落形态的分离物中，每种表型也表现出显著的差异。尽管分离株是单一克隆谱系的一部分，但在表型上表现出很大程度的个性，这表明囊性纤维化呼吸道中的铜绿假单胞菌种群被严重抽样不足。
The opportunistic pathogen Pseudomonas aeruginosa chronically infects the lower airways of patients with cystic fibrosis. Throughout the course of infection this organism undergoes adaptations that contribute to its long-term persistence in the airways. While P. aeruginosa diversity has been documented, it is less clear to what extent within-patient diversity contributes to the overall population structure as most studies have been limited to the analysis of only a few isolates per patient per time point. To examine P. aeruginosa population structure in more detail we collected multiple isolates from individual sputum samples of a patient chronically colonized with P. aeruginosa. This strain collection, comprised of 169 clonal isolates and representing three pulmonary exacerbations as well as clinically stable periods, was assayed for a wide selection of phenotypes. These phenotypes included colony morphology, motility, quorum sensing, protease activity, auxotrophy, siderophore levels, antibiotic resistance, and growth profiles. Each phenotype displayed significant variation even within isolates of the same colony morphotype from the same sample. Isolates demonstrated a large degree of individuality across phenotypes, despite being part of a single clonal lineage, suggesting that the P. aeruginosa population in the cystic fibrosis airways is being significantly under-sampled.
影响因子：
120.7
作者：
Li, ZH;Kosorok, MR;Splaingard, ML
通讯作者：
Splaingard, ML
DOI：
10.1073/pnas.0602138103
发表时间：
2006-05-30
影响因子：
11.1
作者：
Smith, Eric E.;Buckley, Danielle G.;Olson, Maynard V.
通讯作者：
Olson, Maynard V.
阿奇霉素对囊性纤维化患者铜绿假单胞菌临床分离株的影响。
影响因子：
9.6
作者：
Wagner,Thor;Soong,Grace;Sokol,Sach;Saiman,Lisa;Prince,Alice
通讯作者：
Prince,Alice