Genotyping of Candida albicans by fragment analysis of microsatellites combined with 25S rDNA and RPS-based strategies.
Genotyping of Candida albicans by fragment analysis of microsatellites combined with 25S rDNA and RPS-based strategies.
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DOI：
10.3314/jjmm.50.167
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发表时间：
2009-01-01
期刊：
影响因子：
--
通讯作者：
Kanbe, Toshio
中科院分区：
文献类型：
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作者：
Adachi, Hidesada;Shimizu, Kazue;Kanbe, Toshio
微卫星片段分析因其高度的区分能力而被广泛应用于白念珠菌的基因分型。为了评价微卫星片段分析结合25SrDNA和RPS PCR的基因分型系统，对456株独立的C.采用4个微卫星标记（CDC3、HIS3、CA I和CA III）进行基因型分析，然后进行基于25S rDNA和RPS的基因分型。使用CA I的片段分析显示最高的区分潜力（DP=0.9782），其次是HIS 3（DP=0.8780）。利用组合微卫星标记，456 C.白念珠菌分为384个基因型（DP=0.9984）。以25SrDNA和RPS为靶点进行PCR，以区分在片段分析中显示相同基因型的菌株，产生434种基因型（DP=0.9996）。联合基因分型系统在菌株水平上显示出较高的区分能力，因此可用于念珠菌病分子流行病学研究中的快速基因分型。
Because of its high discriminatory potential, fragment analysis of microsatellites has been frequently used for genotyping of Candida albicans at the strain level. In order to evaluate a genotyping system based on the fragment analysis of microsatellites combined with PCRs targeting 25S rDNA and RPS, 456 independent strains of C. albicans were subjected to genotype analysis using 4 microsatellite markers (CDC3, HIS3, CA I and CA III), followed by 25S rDNA and RPS-based genotyping. The fragment analysis using CA I showed the highest discriminatory potential (DP=0.9782), followed by HIS3 (DP=0.8780). Using combined microsatellite markers, 456 C. albicans strains were divided into 384 genotypes (DP=0.9984). PCRs targeting 25S rDNA and RPS were performed to differentiate the strains that showed identical genotypes in the fragment analysis, resulting in 434 genotypes (DP=0.9996). The combined genotyping system showed high discriminatory power at the strain level, and therefore is useful for rapid genotyping in molecular epidemiological studies of candidiasis.