Manner of interaction of heterogeneous claudin species within and between tight junction strands.
Manner of interaction of heterogeneous claudin species within and between tight junction strands.
复制标题
紧密连接链内和之间异质claudin物种相互作用的方式。
DOI：
10.1083/jcb.147.4.891
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发表时间：
1999-11-15
影响因子：
7.8
通讯作者：
Tsukita, S
中科院分区：
文献类型：
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作者：
Furuse, M;Sasaki, H;Tsukita, S
在紧密连接（TJs）中，TJ链在侧面与相邻细胞的链相连，形成成对的链以消除细胞外间隙。紧密连接的整合膜蛋白克劳丁 - 1和 - 2在转染到L成纤维细胞中时可重构成对的TJ链。克劳丁蛋白包含一个多基因家族，并且在单个细胞中会共表达两种以上不同的克劳丁蛋白，这就引出了以下问题：不同的克劳丁蛋白是否会形成异源多聚体TJ链，以及克劳丁蛋白是否以异嗜性方式在成对链之间相互作用。为了回答这些问题，我们将克劳丁 - 1、 - 2和 - 3中的两种共转染到L细胞中，并检测到它们在细胞 - 细胞边界处集中形成精细的网络。免疫复制电子显微镜证实不同的克劳丁蛋白共同整合到单个TJ链中。接下来，将分别单独表达克劳丁 - 1、 - 2或 - 3的两种L转染细胞共培养，我们发现克劳丁 - 3链在侧面与克劳丁 - 1和 - 2链相连形成成对的链，而克劳丁 - 1链不与克劳丁 - 2链相互作用。我们得出结论，除了某些组合外，不同种类的克劳丁蛋白可以在TJ链内部以及链之间相互作用。克劳丁蛋白的这种组装模式可以增加TJ链结构和功能的多样性。
In tight junctions (TJs), TJ strands are associated laterally with those of adjacent cells to form paired strands to eliminate the extracellular space. Claudin-1 and -2, integral membrane proteins of TJs, reconstitute paired TJ strands when transfected into L fibroblasts. Claudins comprise a multigene family and more than two distinct claudins are coexpressed in single cells, raising the questions of whether heterogeneous claudins form heteromeric TJ strands and whether claudins interact between each of the paired strands in a heterophilic manner. To answer these questions, we cotransfected two of claudin-1, -2, and -3 into L cells, and detected their coconcentration at cell–cell borders as elaborate networks. Immunoreplica EM confirmed that distinct claudins were coincorporated into individual TJ strands. Next, two L transfectants singly expressing claudin-1, -2, or -3 were cocultured and we found that claudin-3 strands laterally associated with claudin-1 and -2 strands to form paired strands, whereas claudin-1 strands did not interact with claudin-2 strands. We concluded that distinct species of claudins can interact within and between TJ strands, except in some combinations. This mode of assembly of claudins could increase the diversity of the structure and functions of TJ strands.