Actions of peptides isolated from amphibian skin on pancreatic acinar cells.
Actions of peptides isolated from amphibian skin on pancreatic acinar cells.
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从两栖动物皮肤中分离出的肽对胰腺腺泡细胞的作用。
DOI：
10.1152/ajpendo.1978.235.2.e112
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
J. Gardner
中科院分区：
文献类型：
--
作者：
R. May;T. Conlon;V. Erspamer;J. Gardner
在从豚鼠胰腺制备的分散腺泡细胞中，从两栖动物皮肤中分离的肽（毛蛋白、树蜂素、litorin和physalaemin）和eledoisin（一种从地中海章鱼的后唾腺中分离的肽）增加了45Ca的流出、结合的45Ca的释放、环GMP的积累和淀粉酶的释放。此外，bombesin、litorin、physalaemin和eledoisin均增加了分散的腺泡细胞对45Ca的初始摄取，而猪胆囊收缩素（CCK-OP）和氨甲酰胆碱的c端八肽并没有增加45Ca的初始摄取，反而抵消了其他药物引起的45Ca的增加。阿托品浓度足以消除毒蕈碱类胆碱能药物的作用，但这些两栖动物肽的任何作用都没有改变。两栖动物肽没有改变细胞环AMP或分泌素或猪血管活性肠肽（VIP）引起的增加。与45Ca +炸弹蛋白预孵育的腺泡细胞显示出与对照细胞相同的45Ca释放率，并且该速度没有因加入炸弹蛋白而改变，但通过加入CCK-OP增加了五倍。根据它们的化学结构以及它们改变腺泡细胞功能的效力和功效，两栖动物多肽加CCK-OP可以分为三对：caerulein加CCK-OP， bombesin加litorin， physalaemin加eledoisin。
In dispersed acinar cells prepared from guinea pig pancreas, peptides isolated from amphibian skin (caerulein, bombesin, litorin, and physalaemin) as well as eledoisin, a peptide isolated from the posterior salivary gland of a Mediterranean octopod, increased outflux of 45Ca, release of bound 45Ca, accumulation of cyclic GMP, and release of amylase. In addition, bombesin, litorin, physalaemin, and eledoisin each increased the initial uptake of 45Ca by dispersed acinar cells, whereas C-terminal octapeptide of porcine cholecystokinin (CCK-OP) and carbamylcholine did not increase the initial uptake of 45Ca but, rather, abolished the increase caused by the other agents. None of the actions of these amphibian peptides was altered by concentrations of atropine sufficient to abolish the effects of muscarinic cholinergic agents. None of the amphibian peptides altered cellular cyclic AMP or the increase caused by secretin or porcine vasoactive intestinal peptide (VIP). Acinar cells preincubated with 45Ca plus bombesin showed the same rate of release of 45Ca as did control cells and this rate was not altered by adding bombesin but was increased fivefold by adding CCK-OP. In terms of their chemical structures as well as the potency and efficacy with which they alter acinar cell function, the amphibian peptides plus CCK-OP can be grouped into three pairs: caerulein with CCK-OP, bombesin with litorin, and physalaemin with eledoisin.