The inhibition by valproic acid of the mitochondrial oxidation of monocarboxylic and omega-hydroxymonocarboxylic acids: possible implications for the metabolism of gamma-aminobutyric acid.
The inhibition by valproic acid of the mitochondrial oxidation of monocarboxylic and omega-hydroxymonocarboxylic acids: possible implications for the metabolism of gamma-aminobutyric acid.
复制标题
丙戊酸对单羧酸和 omega-羟基单羧酸线粒体氧化的抑制：对 γ-氨基丁酸代谢的可能影响。
DOI：
10.1093/oxfordjournals.jbchem.a122036
复制
发表时间：
1987
影响因子：
2.7
通讯作者：
Vamecq,J
中科院分区：
文献类型：
--
作者：
Draye,JP;Vamecq,J
材料和方法材料-高香草酸和过氧化物酶II购自Sigma Chemical Co.(圣路易斯，密苏里州，美国)。FAD、CoA、NAD、ADP和ATP来自Boehringer Pharma(BRD曼海姆)。棕榈酸、月桂酸和丁酸的辅酶A和肉碱衍生物来自Pharmacia(瑞典乌普萨拉)。丙戊酸、棕榈酸、月桂酸、癸酸、辛酸、丁酸、16-羟基苯丙酸、12-羟基月桂酸和4-羟基丁酸分别来自Janssen Chemica(比利时Beerse)和Aldrich Chemie(比利时布鲁塞尔)。铁氰化钾购自默克公司(Darmstadt，BRD)。动物-成年雄性白化Wistar大鼠(200-300g)以标准实验动物饲料喂养。丙戊酸盐处理的动物饲喂固体饲料，固体饲料是将动物饲料粉末与0.5%或0.1%(w/w)的中和丙戊酸混合制成的。氯贝特处理的大鼠接受含有0.5%(w/w)氯贝特的饮食。
MATERIALS AND METHODSMaterials-Homovanillic acid and peroxidase Type II were purchased from Sigma Chemical Co.(St. Louis, Mo., USA). FAD, CoA, NAD, ADP, and ATP were from Boehringer Pharma (Mannheim, BRD). CoA and carnitine deriva tives of palmitic, lauric, and butyric acids were obtained from Pharmacia (Uppsala, Sweden). Valproic, palmitic, lauric, decanoic, octanoic, butyric, 16-hydroxypalmitic, 12-hydroxylauric, and 4-hydroxybutyric acids were from Janssen Chemica (Beerse, Belgium) and 10-hydroxydecanoic acid from Aldrich Chemie (Brussels, Belgium). Potas sium ferricyanide was purchased from Merck (Darmstadt, BRD).Animals-Adult male albino Wistar rats (200-300 g) were fed on a standard laboratory animal chow. Valproate-treated animals were fed on a solid diet made by mixing the powdered animal chow with either 0.5 or 0.1%(w/w) neutralized valproic acid. Clofibrate-treated rats received a 0.5%(w/w) clofibrate-containing diet.