Serum immune responses in common carp (Cyprinus carpio L.) to paraquat exposure: The traditional parameters and circulating microRNAs
Serum immune responses in common carp (Cyprinus carpio L.) to paraquat exposure: The traditional parameters and circulating microRNAs
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DOI：
10.1016/j.fsi.2018.02.046
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发表时间：
2018-05
影响因子：
4.7
通讯作者：
Junguo Ma;Yuanyuan Li;Mengli Wu;Can Zhang;Yuqing Che;Weiguo Li;Xiaoyu Li
中科院分区：
文献类型：
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作者：
Junguo Ma;Yuanyuan Li;Mengli Wu;Can Zhang;Yuqing Che;Weiguo Li;Xiaoyu Li
百草枯（PQ）是一种世界范围内广泛使用的除草剂，对水生生物是一种高风险化合物。本研究旨在探讨PQ对鲤鱼传统血清指标和循环microRNAs（microRNAs，miRNAs）的影响，以进一步阐明PQ对鱼类的毒性机制。在实验室条件下，采用1.596和3.192 mg/L的PQ对鲤鱼进行了连续7 d的亚急性毒性试验。结果表明，PQ暴露可显著降低鱼血清中总抗氧化能力（T-AOC）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽硫转移酶（GST）的含量，但显著增加了丙二醛（MDA）的含量，表明PQ暴露可诱导鱼体内氧化应激和脂质过氧化反应。生化检测结果显示，PQ暴露不仅显著改变了LDH、AST、ALT、ACP、AKP和溶菌酶的活性以及IgM和补体3的含量，还促进了IFN-γ、IL-1β、IL-6、IL-8和TNF-α等促炎细胞因子的表达。此外，PQ还抑制了抗炎性细胞因子IL-10和TGF-β的水平，提示PQ暴露可能会导致鱼类组织损伤并促进免疫炎症反应。此外，我们发现，血清循环miRNA，如ccr-mir-122，ccr-mir-125 b，ccr-mir-146 a和ccr-mir-155，通常在PQ暴露后的鱼中得到促进。基于我们的结果和基于miRNA的哺乳动物组织损伤和炎症反应诊断的报告，我们建议血清ccr-mir-122，ccr-mir-125 b，ccr-mir-146 a和ccr-mir-155可能是PQ毒性的新生物标志物。
Paraquat (PQ) is a herbicide used worldwide, and it was shown to be a high-risk compound to aquatic organisms. This study was conducted to investigate the effects of PQ on traditional serum parameters and circulating microRNAs (miRNAs) in common carp to further elucidate the mechanism of PQ toxicity in fish. In the current study, a subacute toxicity test of common carp exposed to PQ at 1.596 and 3.192 mg/L for 7 d was conducted under laboratory conditions. The results showed that PQ exposure generally reduced the levels of T-AOC, SOD, CAT, and GST, but significantly increased MDA levels in the serum, indicating that PQ exposure induces oxidative stress and lipid peroxidation in the fish. The results of biochemical assays showed that PQ exposure not only significantly altered the activities of LDH, AST, ALT, ACP, AKP, and lysozyme and the contents of IgM and complement 3 but also promoted the expression of pro-inflammatory cytokines, including IFN-γ, IL-1β, IL-6, IL-8, and TNF-α. Additionally, PQ inhibited the levels of the anti-inflammatory cytokines IL-10 and TGF-β, suggesting that PQ exposure may cause fish tissue injury and promote immune inflammatory responses. Furthermore, we found that serum circulating miRNAs, such as ccr-mir-122, ccr-mir-125b, ccr-mir-146a, and ccr-mir-155, were generally promoted in fish following PQ exposure. Based on our results and reports on miRNA-based diagnosis of tissue damage and inflammatory responses in mammals, we suggest that serum ccr-mir-122, ccr-mir-125b, ccr-mir-146a, and ccr-mir-155 could be new biomarkers of PQ toxicity in fish.