Reduced migration of Ishikawa cells associated with downregulation of aquaporin-5
Reduced migration of Ishikawa cells associated with downregulation of aquaporin-5
复制标题
与 aquaporin-5 下调相关的 Ishikawa 细胞迁移减少
DOI：
10.3892/ol.2012.738
复制
发表时间：
2012-08-01
期刊：
影响因子：
2.9
通讯作者：
Wu, Rui Jin
中科院分区：
文献类型：
--
作者：
Jiang, Xiu Xiu;Xu, Kai Hong;Wu, Rui Jin
水通道蛋白（AQP）依赖的细胞迁移在血管生成、肿瘤转移、伤口愈合、胶质瘢痕形成和其他需要细胞运动的事件中具有广泛的意义。在哺乳动物中已鉴定出13种AQP亚型（0-12）。目前尚不清楚AQP 5是否在子宫内膜癌的发展中起作用。我们最近发现，卵巢类固醇激素可能会影响女性生殖道中AQP 5的表达。在这项研究中，我们考虑是否AQP 5可能会影响细胞迁移的石川细胞，腺癌细胞系来自子宫内膜。结果表明，AQP 5的下调导致石川细胞迁移减少。在正常子宫内膜和子宫内膜癌中，AQP 5启动子区的E2受体介导了AQP 5表达的调节。相比之下，E2上调AQP 5通过增加膜联蛋白2增加细胞迁移、侵袭和粘附，膜联蛋白2负责F-肌动蛋白重塑和重排。E2通过调节AQP 5的表达来调节石川细胞的迁移。
Aquaporin (AQP)-dependent cell migration has broad implications in angiogenesis, tumor metastasis, wound healing, glial scarring and other events requiring cell movement. There are 13 isoforms of AQP (0-12) that have been identified in mammals. It is unclear whether AQP5 plays a role in the development of endometrial cancer. We recently demonstrated that ovarian steroids may affect the expression of AQP5 in the female genital tract. In this study, we considered whether AQP5 may affect cell migration in Ishikawa cells, an adenocarcinoma cell line derived from the endometrium. The results showed that the downregulation of AQP5 results in reduced Ishikawa cell migration. The estrogen (E2) receptor in the promoter of AQP5 mediated the regulation of AQP5 expression in the normal endometrium and endometrial cancer. By contrast, the upregulation of AQP5 by E2 increased cell migration, invasion and adhesion through increased annexin-2, which is responsible for F-actin remodeling and rearrangement. E2 regulates Ishikawa cell migration by regulating the AQP5 expression.