Electrochemically reduced water exerts superior reactive oxygen species scavenging activity in HT1080 cells than the equivalent level of hydrogen-dissolved water.
Electrochemically reduced water exerts superior reactive oxygen species scavenging activity in HT1080 cells than the equivalent level of hydrogen-dissolved water.
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DOI：
10.1371/journal.pone.0171192
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Shirahata S
中科院分区：
文献类型：
--
作者：
Hamasaki T;Harada G;Nakamichi N;Kabayama S;Teruya K;Fugetsu B;Gong W;Sakata I;Shirahata S
电化学还原水（ERW）在电解过程中在阴极附近产生，并且呈现碱性pH，含有丰富溶解的氢，并且含有少量铂纳米颗粒。战争遗留爆炸物具有清除活性氧的活性，最近的研究表明，溶于氢的水具有清除活性氧的活性。因此，据推测，战争遗留爆炸物的抗氧化能力取决于氢的含量;然而，没有任何报告证实溶解的氢在战争遗留爆炸物中的作用。在本报告中，我们澄清了战争遗留爆炸物中抗氧化活性的响应因素是否是溶解氢。采用荧光染色法和免疫自旋捕获法检测了ERW和氢溶水对细胞内ROS的清除作用。我们证实，ERW具有电解强度依赖的细胞内ROS-清除活性，ERW在HT 1080细胞中发挥显着上级ROS-清除活性比同等水平的氢溶解水。战争遗留爆炸物在去除溶解氢后仍保持清除活性氧的活性，但在高压灭菌后失去活性。氧自由基吸收能力测定法、2，2-二苯基-1-苦肼测定法和化学发光测定法均不能检测出战争遗留爆炸物和溶氢水中的自由基清除活性。这些结果表明，战争遗留爆炸物含有电解依赖的氢和一个额外的抗氧化因子预测铂纳米粒子。
Electrochemically reduced water (ERW) is produced near a cathode during electrolysis and exhibits an alkaline pH, contains richly dissolved hydrogen, and contains a small amount of platinum nanoparticles. ERW has reactive oxygen species (ROS)-scavenging activity and recent studies demonstrated that hydrogen-dissolved water exhibits ROS-scavenging activity. Thus, the antioxidative capacity of ERW is postulated to be dependent on the presence of hydrogen levels; however, there is no report verifying the role of dissolved hydrogen in ERW. In this report, we clarify whether the responsive factor for antioxidative activity in ERW is dissolved hydrogen. The intracellular ROS scavenging activity of ERW and hydrogen-dissolved water was tested by both fluorescent stain method and immuno spin trapping assay. We confirm that ERW possessed electrolysis intensity-dependent intracellular ROS-scavenging activity, and ERW exerts significantly superior ROS-scavenging activity in HT1080 cells than the equivalent level of hydrogen-dissolved water. ERW retained its ROS-scavenging activity after removal of dissolved hydrogen, but lost its activity when autoclaved. An oxygen radical absorbance capacity assay, the 2,2-diphenyl-1-picrylhydrazyl assay and chemiluminescence assay could not detect radical-scavenging activity in both ERW and hydrogen-dissolved water. These results indicate that ERW contains electrolysis-dependent hydrogen and an additional antioxidative factor predicted to be platinum nanoparticles.