THE DEVELOPMENT OF PLASMODIUM OOKINETES INVITRO - AN ULTRASTRUCTURAL-STUDY INCLUDING A DESCRIPTION OF MEIOTIC DIVISION
THE DEVELOPMENT OF PLASMODIUM OOKINETES INVITRO - AN ULTRASTRUCTURAL-STUDY INCLUDING A DESCRIPTION OF MEIOTIC DIVISION
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DOI：
10.1017/s0031182000057334
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发表时间：
1985-01-01
期刊：
影响因子：
2.4
通讯作者：
WINGER, L
中科院分区：
文献类型：
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作者：
SINDEN, RE;HARTLEY, RH;WINGER, L
用改进的韦斯和范德伯格（1977）的方法在体外培养动合子。通过在pH 8.4的培养基中培养和血液稀释度为1/10或超过1/10，产生了技术上的显著改进。用膜饲养技术生产的动合子对蚊虫具有感染性。超微结构分析的核，细胞骨架，晶体和微线的发展。受精卵的第一次核内分裂被认为是减数分裂。染色体在分裂前期的浓缩过程遵循典型的细线期、偶线期和粗线期。双线期和终变期不存在，联会复合体持续到中期I。染色体在分裂后期分离，并在分裂末期之前迅速解聚。在动合子中我们还没有发现第二次减数分裂。这些研究结果的分子和孟德尔组织的寄生虫基因组的影响进行了讨论。
Ookinetes have been cultured in vitro using modifications to the method of Weiss and Vanderberg (1977). Significant improvements in technique were produced by culture in medium at pH 8.4 and at a blood dilution at or over 1/10. Ookinetes produced were infective to mosquitoes by membrane feeding techniques. Ultrastructural analyses were made of nuclear, cytoskeletal, crystalloid and microneme development. The first intranuclear division in the zygote has been recognized as meiosis. Chromosome condensation during prophase follows the classical stages of leptotene, zygotene and pachytene. Diplotene and diakinesis are not present-the synaptonemal complexes persist into metaphase I. Chromosomes separate at anaphase and rapidly de-condense prior to telophase. We have not recognized a second meiotic division in the ookinete. The implication of these findings to the molecular and Mendelian organization of the parasite genome are discussed.