Human T cell subpopulations defined by a monoclonal antibody. I. A small subset is responsible for proliferation to allogeneic cells or to soluble antigens and for helper activity for B cell differentiation.
Human T cell subpopulations defined by a monoclonal antibody. I. A small subset is responsible for proliferation to allogeneic cells or to soluble antigens and for helper activity for B cell differentiation.
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由单克隆抗体定义的人类 T 细胞亚群。
DOI：
10.4049/jimmunol.128.1.16
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发表时间：
1982
影响因子：
4.4
通讯作者：
A. Bargellesi
中科院分区：
文献类型：
--
作者：
G. Corte;M. Mingari;A. Moretta;G. Damiani;L. Moretta;A. Bargellesi
获得了与大多数同种异体活化的人T细胞反应但仅与15 - 20%的外周T细胞反应的单克隆抗体（5/9）。使用5/9包被的ORBC通过玫瑰花结技术将与5/9抗体反应的群体与剩余的T细胞分离。分析5/9+和5/9-群体的不同体外活性。PWM驱动的B细胞分化的辅助活性在5/9+群体中似乎受到限制并高度富集。此外，5/9+细胞含有能够增殖为破伤风类毒素和同种异体细胞的所有细胞。因此，通常用于评价T细胞功能的体外活性似乎仅限于外周T细胞的一小部分。
A monoclonal antibody (5/9) was obtained that reacts with the majority of alloactivated human T cells but only with 15 to 20% peripheral T cells. The populations reactive with the 5/9 antibody were separated from the remaining T cells by rosetting techniques using 5/9-coated ORBC. 5/9+ and 5/9- populations were analyzed for different in vitro activities. The helper activity of PWM- driven B cell differentiation appeared to be restricted and highly enriched in the 5/9+ population. In addition, 5/9+ cells contained all the cells capable of proliferation to tetanus toxoid and allogeneic cells. Thus, in vitro activities commonly used for evaluation of T cell function seem to be confined to a small fraction of peripheral T cells.