DETERMINATION OF ALPHA-1-ANTITRYPSIN PHENOTYPES AND M-SUBTYPES BY ISOELECTRIC-FOCUSING IN IMMOBILIZED PH GRADIENTS
DETERMINATION OF ALPHA-1-ANTITRYPSIN PHENOTYPES AND M-SUBTYPES BY ISOELECTRIC-FOCUSING IN IMMOBILIZED PH GRADIENTS
复制标题
DOI：
10.1016/s0009-9120(85)80032-1
复制
发表时间：
1985-01-01
影响因子：
2.8
通讯作者：
IAMMARINO, RM
中科院分区：
文献类型：
--
作者：
BROOKS, KP;IAMMARINO, RM
一个程序来确定α-1-抗胰蛋白酶表型（Pi型），包括六个常见的PiM亚型，描述。抑制剂的表型，包括缺陷表型PiZ和PiM亚型，可以通过在pH范围为4.2至5.0或4.0至5.0的固定pH梯度中等电聚焦来鉴定。该方法给出了M、S、V、P和Z等位基因产物的清楚分离。使用市售的设备和试剂，一次过夜运行最多可分析24个样品。
A procedure to determine alpha-1-antitrypsin phenotypes (Pi types), including the six common PiM subtypes, is described. Phenotypes of the inhibitor, including the deficiency phenotype PiZ and the PiM subtypes, can be identified by isoelectric focusing in an immobilized pH gradient with a pH range of 4.2 to 5.0 or 4.0 to 5.0. This method gives clear separation of M, S, V, P and Z allele products. Up to 24 samples can be analyzed in a single overnight run using commercially avaible equipment and reagents.