Arabidopsis GDSL1 overexpression enhances rapeseed Sclerotinia sclerotiorum resistance and the functional identification of its homolog in Brassica napus
Arabidopsis GDSL1 overexpression enhances rapeseed Sclerotinia sclerotiorum resistance and the functional identification of its homolog in Brassica napus
复制标题
拟南芥GDSL1过表达增强油菜核盘菌抗性及其同系物在甘蓝型油菜中的功能鉴定
DOI：
10.1111/pbi.13289
复制
发表时间：
2019-11-20
影响因子：
13.8
通讯作者：
Tan, Xiao-Li
中科院分区：
文献类型：
--
作者：
Ding, Li-Na;Li, Ming;Tan, Xiao-Li
油菜菌核病（Sclerotinia stem rot，SSR）是由油菜菌核病（Sclerotinia sclerotiorum）引起的一种毁灭性病害。迄今为止，油菜与S.核盘菌尚未完全了解，分子育种仍然是最有效的控制策略。在此，拟南芥GDSL 1被表征为在核盘菌抗性中起作用的胞外GDSL脂肪酶基因。AtGDSL 1功能缺失导致对S.菌核。相反，在B.油菜的抗性增强与活性氧（ROS）和水杨酸（SA）水平的增加以及茉莉酸水平的降低有关。此外，AtGDSL 1可以引起脂质前体磷脂酸水平的增加，这可能导致下游ROS/SA防御相关通路的激活。而与AtGDSL 1序列相似性最高的BnGDSL 1对SSR抗性没有影响。候选基因关联研究表明，只有一个AtGDSL 1同源油菜，BnaC 07 g35650 D（BnGLIP 1），显着贡献的天然B的抗性性状。napus群体中表达，并通过烟草叶片中的瞬时表达试验证实了抗性功能。基因组分析表明，BnGLIP 1位点在育种过程中嵌入了与SSR抗性相关的选择区域，其优势等位基因类型属于群体中的次要等位基因。因此，BnGLIP 1是AtGDSL 1的功能等价物，在油菜S中具有广泛的应用。抗菌核病育种
Sclerotinia stem rot (SSR) caused by Sclerotinia sclerotiorum is a devastating disease of rapeseed (Brassica napus L.). To date, the genetic mechanisms of rapeseed' interactions with S. sclerotiorum are not fully understood, and molecular-based breeding is still the most effective control strategy for this disease. Here, Arabidopsis thaliana GDSL1 was characterized as an extracellular GDSL lipase gene functioning in Sclerotinia resistance. Loss of AtGDSL1 function resulted in enhanced susceptibility to S. sclerotiorum. Conversely, overexpression of AtGDSL1 in B. napus enhanced resistance, which was associated with increased reactive oxygen species (ROS) and salicylic acid (SA) levels, and reduced jasmonic acid levels. In addition, AtGDSL1 can cause an increase in lipid precursor phosphatidic acid levels, which may lead to the activation of downstream ROS/SA defence-related pathways. However, the rapeseed BnGDSL1 with highest sequence similarity to AtGDSL1 had no effect on SSR resistance. A candidate gene association study revealed that only one AtGDSL1 homolog from rapeseed, BnaC07g35650D (BnGLIP1), significantly contributed to resistance traits in a natural B. napus population, and the resistance function was also confirmed by a transient expression assay in tobacco leaves. Moreover, genomic analyses revealed that BnGLIP1 locus was embedded in a selected region associated with SSR resistance during the breeding process, and its elite allele type belonged to a minor allele in the population. Thus, BnGLIP1 is the functional equivalent of AtGDSL1 and has a broad application in rapeseed S. sclerotiorum-resistance breeding.