A survey on bacteria inhabiting the sea surface microlayer of coastal ecosystems
A survey on bacteria inhabiting the sea surface microlayer of coastal ecosystems
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DOI：
10.1016/j.femsec.2005.04.002
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发表时间：
2005-10-01
影响因子：
4.2
通讯作者：
Lebaron, P
中科院分区：
文献类型：
--
作者：
Agogué, H;Casamayor, EO;Lebaron, P
用培养和遗传指纹分析的方法，对地中海沿岸两个不同站点(污染和贫营养化)的表层微层细菌种群进行了检测，并与底层水域(50 cm深度)进行了为期两年的比较。对30多份样品进行了检测，共分离和筛选出487株细菌。在原始环境中，变形杆菌的数量一直较多，而革兰氏阳性细菌(即放线杆菌和菲尔米特)在污染场地中的数量较多。嗜胞菌-黄杆菌-类杆菌(CFB)占菌株总数的8%~16%。总体而言，22.5%的菌株与已报道的细菌种仅在属水平上具有相似性，约10.5%的菌株与已报道的种的16S rRNA序列相似性在93%以下。CFB组含有最高比例的未知物种，但这些也包括阿尔法和伽马变形杆菌。如此低的相似度表明我们能够培养出新的海洋属，可能还有新的科，这表明海表层是一个知之甚少的微生物环境，可能代表着新微生物的自然来源。然而，遗传指纹图谱显示，表层微层和底层水域的优势细菌组合之间没有一致的差异，这表明稳定和丰富的中性细菌群落的存在并不是沿海海洋环境的共同特征。(C)2005年欧洲微生物学会联合会。爱思唯尔出版，版权所有。
Bacterial populations inhabiting the sea surface microlayer from two contrasted Mediterranean coastal stations (polluted vs. oligotrophic) were examined by culturing and genetic fingerprinting methods and were compared with those of underlying waters (50 cm depth), for a period of two years. More than 30 samples were examined and 487 strains were isolated and screened. Proteobacteria were consistently more abundant in the collection from the pristine environment whereas Gram-positive bacteria (i.e., Actinobacteria and Firmicutes) were more abundant in the polluted site. Cythophaga-Flavobacter-Bacteroides (CFB) ranged from 8% to 16% of total strains. Overall, 22.5% of the strains showed a 16S rRNA gene sequence similarity only at the genus level with previously reported bacterial species and around 10.5% of the strains showed similarities in 16S rRNA sequence below 93% with reported species. The CFB group contained the highest proportion of unknown species, but these also included Alpha- and Gammaproteobacteria. Such low similarity values showed that we were able to culture new marine genera and possibly new families, indicating that the sea-surface layer is a poorly understood microbial environment and may represent a natural source of new microorganisms. Genetic fingerprinting showed, however, no consistent differences between the predominant bacterial assemblages from surface microlayer and underlying waters, suggesting that the presence of a stable and abundant neustonic bacterial community is not a common trait of coastal marine environments. (C) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.