Dynamic Hubs Show Competitive and Static Hubs Non-Competitive Regulation of Their Interaction Partners
Dynamic Hubs Show Competitive and Static Hubs Non-Competitive Regulation of Their Interaction Partners
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DOI：
10.1371/journal.pone.0048209
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发表时间：
2012-10-31
期刊：
影响因子：
3.7
通讯作者：
Wilkins, Marc R.
中科院分区：
文献类型：
--
作者：
Goel, Apurv;Wilkins, Marc R.
Date hub蛋白有1或2个相互作用界面，但有许多相互作用伙伴。这就提出了一个问题，是否所有的伴侣蛋白都竞争枢纽的相互作用界面，或者细胞是否仔细地调节了这一过程的各个方面？在这里，我们使用实时渲染的蛋白质相互作用网络来分析所有的1或2个接口枢纽的酿酒酵母在细胞周期期间的相互作用。通过整合先前确定的结构和基因表达数据，并在表达低谷期间视觉上隐藏节点（蛋白质）及其边缘（相互作用），我们预测何时可能存在枢纽及其合作伙伴的相互作用。这揭示了酵母相互作用组中所有36个单界面或双界面枢纽中的20个属于两个主要群体。第一种是有静态合作伙伴的动态枢纽，可被视为“竞争性枢纽”。它们的相互作用伙伴将竞争枢纽的相互作用界面，并且任何相互作用的成功将由相互作用的动力学（丰度和亲和力）和亚细胞定位决定。第二种是静态枢纽与动态合作伙伴，我们称之为“非竞争性枢纽”。调节机制被精细地调整以减少枢纽的交互伙伴之间的竞争的存在和/或影响。这些调节过程也可能被细胞用于调节其他非细胞周期过程。
Date hub proteins have 1 or 2 interaction interfaces but many interaction partners. This raises the question of whether all partner proteins compete for the interaction interface of the hub or if the cell carefully regulates aspects of this process? Here, we have used real-time rendering of protein interaction networks to analyse the interactions of all the 1 or 2 interface hubs of Saccharomyces cerevisiae during the cell cycle. By integrating previously determined structural and gene expression data, and visually hiding the nodes (proteins) and their edges (interactions) during their troughs of expression, we predict when interactions of hubs and their partners are likely to exist. This revealed that 20 out of all 36 one-or two-interface hubs in the yeast interactome fell within two main groups. The first was dynamic hubs with static partners, which can be considered as 'competitive hubs'. Their interaction partners will compete for the interaction interface of the hub and the success of any interaction will be dictated by the kinetics of interaction (abundance and affinity) and subcellular localisation. The second was static hubs with dynamic partners, which we term 'non-competitive hubs'. Regulatory mechanisms are finely tuned to lessen the presence and/or effects of competition between the interaction partners of the hub. It is possible that these regulatory processes may also be used by the cell for the regulation of other, non-cell cycle processes.