Comparison of Phytochemical and Antioxidant Activities in Micropropagated and Seed-derived Salvia miltiorrhiza Plants
Comparison of Phytochemical and Antioxidant Activities in Micropropagated and Seed-derived Salvia miltiorrhiza Plants
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微繁丹参和种子丹参植物的植物化学和抗氧化活性比较
DOI：
10.21273/hortsci13072-18
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发表时间：
2018-07-01
期刊：
影响因子：
1.9
通讯作者：
Jiang, Yan-Hong
中科院分区：
文献类型：
--
作者：
Liu, Bo-Ling;Fan, Zhi-Bin;Jiang, Yan-Hong
丹参（Salvia miltiorrhiza，中国俗称丹参）是一种常用的中药，用于治疗多种疾病，尤其是心血管疾病。丹参的商业繁殖通过种子萌发或体外再生（微繁殖）进行。然而，目前尚不清楚不同的繁殖方法是否会影响衍生植物的化学性质。本研究首次建立了丹参高效组培繁殖体系。MS培养基中添加1.0 mg·L-1的6-苄基腺嘌呤（BA）和0.1 mg·L-1的α-萘乙酸（NAA）对不定芽的诱导效果最好，MS +0.2 mg·L-1的NAA生根率最高，移栽成活率达95%。接下来，我们评估了相同年龄的植物的不同组织中的抗氧化性能，来自微繁殖或种子萌发，并测量丹参酮，总酚，总黄酮含量。结果表明，组培苗组织的抗氧化活性高于种子苗组织;组培苗根中丹参酮的含量也高于种子苗。本研究建立了丹参快速高效的离体繁殖体系，为丹参的培养和药用化合物的获得奠定了基础。
Salvia miltiorrhiza (commonly known in China as Danshen) is widely used in traditional Chinese medicine, and it is applied in the treatment of many diseases, particularly cardiovascular disease. Commercial propagation of Danshen is carried out either through seed germination or in vitro regeneration (micropropagation). However, it is not clear if the different propagation methods affect the chemical properties of the derived plants. In the present study, we first established a highly efficient tissue culture system for Danshen propagation. The addition of 1.0 mg·L−1 6-benzyladenine (BA) and 0.1 mg·L−1 α-naphthalene acetic acid (NAA) to Murashige and Skoog (MS) medium was optimal for inducing adventitious shoots; the highest rate of rooting was recorded on MS medium with 0.2 mg·L−1 NAA, on which the survival rate of transplanted plantlets was 95%. Next, we assessed antioxidant properties in the different tissues of plants of the same age, derived from micropropagation or seed germination, and measured tanshinone, total phenol, and total flavonoid contents. Our results showed that tissues of micropropagated plantlets had higher antioxidant activities than tissues of seed-derived plantlets; the micropropagated plantlets also had higher tanshinone contents in their roots. Thus, a rapid and efficient micropropagation system was established for Danshen, and it can be used for cultivating this plant to obtain therapeutic compounds.