In Vitro Selection Combined with Ribosomal Translation Containing Non-proteinogenic Amino Acids
In Vitro Selection Combined with Ribosomal Translation Containing Non-proteinogenic Amino Acids
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含有非蛋白氨基酸的体外选择与核糖体翻译相结合
DOI：
10.1002/tcr.201500239
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Tomoshige Fujino and Hiroshi Murakami
中科院分区：
文献类型：
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作者：
Fujino;T. Goto;Y. Suga;H. Murakami;H.;Tomoshige Fujino and Hiroshi Murakami
自从将非蛋白质氨基酸引入核糖体翻译系统以来，这些分子的潜在应用不断增加。使用这些分子，已经进行了越来越多的关于主题的研究，例如将人工功能添加到蛋白质、具有人工残基的蛋白质的细胞表达、以及具有新功能的人工肽的开发。在这里，我们描述了最近的研究，阐明了非蛋白质氨基酸与核糖体翻译的相容性。我们还描述了一种简单而高速的选择方法的发展及其在创造具有非蛋白质氨基酸的新型功能肽方面的潜在应用。由于这些研究扩大了人工肽库的多样性，提高了新功能肽的选择速度，它们将显著促进新分子的开发，如药物候选物和生物测定探针。
The potential applications of non‐proteinogenic amino acids have increased continuously since the introduction of these molecules into a ribosomal translation system. An increasing number of studies concerning topics, such as the addition of an artificial function to a protein, cellular expression of a protein with an artificial residue, and development of an artificial peptide with a novel function, have been done using these molecules. Here, we describe recent studies that elucidate the compatibility of non‐proteinogenic amino acids with ribosomal translation. We also describe the development of a simple and high‐speed selection method and its potential application for the creation of a novel functional peptide with non‐proteinogenic amino acids. As these studies have expanded the diversity of the artificial peptide library and increased the speed of novel functional peptide selection, they will significantly facilitate the development of new molecules, such as pharmaceutical drug candidates and bioassay probes.