Expression of specific binding sites on Candida with functional and antigenic characteristics of human complement receptors.
Expression of specific binding sites on Candida with functional and antigenic characteristics of human complement receptors.
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DOI：
10.4049/jimmunol.137.11.3577
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发表时间：
1986-12
影响因子：
4.4
通讯作者：
J. Edwards;T. Gaither;J. O’Shea;D. Rotrosen;T. Lawley;S. Wright;M. Frank;I. Green
中科院分区：
文献类型：
--
作者：
J. Edwards;T. Gaither;J. O’Shea;D. Rotrosen;T. Lawley;S. Wright;M. Frank;I. Green
C3降解片段（CR1、CR2和CR3）的受体存在于包括吞噬细胞和淋巴样细胞在内的许多人类细胞中，并且可能在入侵微生物的附着中起关键作用。在这些研究中发现念珠菌通过结合包被特定人C3片段的红细胞来模拟人的CR。念珠菌种类的酵母菌形式粘附在玻片上并允许发芽。绵羊红细胞(E)包被IgM （EA）和人补体成分，制备EA、EAC14、EAC3b、EAC3bi和EAC3d。然后检查这些测试细胞对生物体的粘附性。使用人CR1、CR2和CR3抗体来评估它们阻断试验红细胞粘附念珠菌的潜力。荧光素标记的人补体受体抗体也被用来表征结合位点。EAC3bi和EAC3d广泛结合在白色念珠菌和stellatoidea念珠菌的胚管和假菌丝上，但不包括E、EA和EAC14。EAC3b不常结合。其他念珠菌种类，通常被认为致病性较低，结合特异性试验红细胞明显少于白色念珠菌。针对人CR1和CR3的单克隆抗体（3D9、1B4、C511、2B6、抗b2、Mo1和抗mac -1）一般不阻断受试红细胞的粘附。高浓度的单克隆（抗cr2） HB-5和多克隆（抗cr2）抗gp 140可阻断EAC3bi和EAC3d试验红细胞粘附少量C3片段。免疫荧光研究表明，Mo-1与生物体的萌发形式结合，而其他抗体的结合未见。这些研究表明，这种生物的表面成分类似于人类细胞的CR。需要进一步的研究来进一步确定结合位点的分子性质。生物模仿人类CR的能力可能比以前所知的更广泛，并且可能作为炎症和免疫反应的一种改变机制。
Receptors for C3 degradation fragments (CR1, CR2, and CR3) are present on many human cells including phagocytes and lymphoid cells and may be critical in the attachment of invading microorganisms. In these studies Candida were found to mimic the human CR by binding erythrocytes coated with specific human C3 fragments. Yeast forms of Candida species were adhered to glass slides and were allowed to germinate. Sheep erythrocytes (E) were coated with IgM (EA) and human complement components to prepare EA, EAC14, EAC3b, EAC3bi, and EAC3d. These test cells were then examined for adherence to the organism. Antibodies to human CR1, CR2, and CR3 were used to evaluate their potential for blocking adherence of the test erythrocytes to Candida. Fluorescein-labeled antibodies to human complement receptors were also used to characterize the binding sites. EAC3bi and EAC3d, but not E, EA, or EAC14, bound extensively to the germ tubes and pseudohyphae of Candida albicans and C. stellatoidea. EAC3b bound infrequently. Other Candida species, generally considered less pathogenic, bound significantly fewer specific test erythrocytes than C. albicans. Monoclonal antibodies to human CR1 and CR3 (3D9, 1B4, C511, 2B6, anti-B2, Mo1, and anti-Mac-1), in general, did not block adherence of test erythrocytes. Blocking of adherence of EAC3bi and EAC3d test erythrocytes coated with small quantities of C3 fragments occurred with high concentrations of monoclonal (anti-CR2) HB-5 and polyclonal (anti-CR2) anti-GP 140. Immunofluorescence studies demonstrated binding of Mo-1 to the germinated forms of the organism, whereas binding of the other antibodies was not seen. These studies suggest a surface constituent on the organism similar to CR on human cells. Additional studies are necessary to further define the molecular nature of the binding site. The ability of organisms to mimic human CR may be more generalized than previously known and may serve as a mechanism for modification of the inflammatory and immune response.