EARLY TRANSCRIBED SEQUENCES AFFECT TERMINATION EFFICIENCY OF ESCHERICHIA-COLI RNA-POLYMERASE
EARLY TRANSCRIBED SEQUENCES AFFECT TERMINATION EFFICIENCY OF ESCHERICHIA-COLI RNA-POLYMERASE
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DOI：
10.1016/0022-2836(89)90344-6
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发表时间：
1989-01-20
影响因子：
5.6
通讯作者：
ROBERTS, JW
中科院分区：
文献类型：
--
作者：
GOLIGER, JA;YANG, XJ;ROBERTS, JW
我们构建了新的转录模板，其中融合了大肠杆菌噬菌体的晚期基因启动子。lambda。82个上游来自三个不同的rho无关转录终止子。通过体外转录实验和体内半乳糖激酶表达实验，我们发现转录区域的初始部分显著影响一些下游终止子的效率。通过缺失、替换和点突变分析，我们已经确定了导致这些增加的因子独立读通水平的序列。由于这些重要的序列发生在RNA起始位点约30个核苷酸内，我们认为转录本的起始部分会影响终止效率。
We have constructed novel transcription templates in which we have fused the late gene promoters of Escherichia coli phages .lambda. and 82 upstream from three different rho-independent transcription terminators. Using an in vitro transcription assay and an in vivo galactokinase expression assay, we find that the initial portion of the transcribed region significantly affects the efficiency of some downstream terminators. We have identified, by deletion, substitution and point mutation analysis, sequences responsible for these increased levels of factor-independent readthrough. Since these important sequences occur within about 30 nucleotides of the RNA start site, we suggest that the initial portion of the trancript can affect termination efficiency.