HIGH-FREQUENCY OF UNEQUAL RECOMBINATION IN PSEUDOAUTOSOMAL REGION SHOWN BY PROVIRAL INSERTION IN TRANSGENIC MOUSE
HIGH-FREQUENCY OF UNEQUAL RECOMBINATION IN PSEUDOAUTOSOMAL REGION SHOWN BY PROVIRAL INSERTION IN TRANSGENIC MOUSE
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DOI：
10.1038/324682a0
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发表时间：
1986-12-18
期刊：
影响因子：
64.8
通讯作者：
JAENISCH, R
中科院分区：
文献类型：
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作者：
HARBERS, K;SORIANO, P;JAENISCH, R
与常染色体相反，哺乳动物的X和Y染色体在雄性减数分裂期间仅在端粒附近配对。位于该区域的等位基因可以在减数分裂1 -4期间进行相互交换。因为这些序列不显示严格的性连锁遗传，它们被称为假常染色体5。在人类中，已经描述了几个DNA序列，它们显示出假常染色体传递，并且位于X和Y染色体短臂末端的配对区（参考文献6-9和D.页，未公布的结果）。我们现在表明，转基因小鼠品系，MOV-15，包含一个单一的莫洛尼鼠白血病病毒（M-MuLV）基因组在其种系，遗传学证据表明，前病毒整合到性染色体的假常染色体区域。在该品系中，7%的雄性减数分裂中前病毒拷贝丢失或获得，并且前病毒侧翼的小鼠序列串联重复且高度可变。我们的结论是不平等的重组事件发生在配对区域的频率很高，可能是因为存在重复序列。
The mammalian X and Y chromosomes, in contrast to the autosomes, pair during male meiosis only near the telomeres. Alleles localized in this region can undergo reciprocal exchange during meiosis1–4. Because such sequences do not show strict sex-linked inheritance, they have been termed pseudoautosomal5. In man, several DNA sequences have been described which show pseudoautosomal transmission and which are localized in the pairing region at the ends of the short arms of both the X and Y chromosomes (refs 6–9, and D. Page, unpublished results). We now show that the transgenic mouse strain, Mov-15, contains a single Moloney murine leukaemia virus (M-MuLV) genome in its germline, and genetic evidence indicates that the provirus is integrated into the pseudoautosomal region of the sex chromosome. Proviral copies are lost or gained in 7% of male meioses in this strain, and mouse sequences flanking the provirus are tandemly repeated and highly variable. We conclude that unequal recombination events occur with high frequency in the pairing region, possibly because of the presence of repeated sequences.