Association mapping of seed oil content in Brassica napus and comparison with quantitative trait loci identified from linkage mapping.
Association mapping of seed oil content in Brassica napus and comparison with quantitative trait loci identified from linkage mapping.
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DOI：
10.1139/g10-075
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发表时间：
2010-11
期刊：
影响因子：
3.1
通讯作者：
J. Zou;Congcong Jiang;Zhengying Cao;Ruiyuan Li;Y. Long;Sheng Chen;J. Meng
中科院分区：
文献类型：
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作者：
J. Zou;Congcong Jiang;Zhengying Cao;Ruiyuan Li;Y. Long;Sheng Chen;J. Meng
关联图谱越来越多地应用于具有丰富遗传多样性的自然群体中，以检测与重要农艺性状相关的dna标记。甘蓝型油菜是一种重要的油料作物，遗传多样性有限。利用近缘种的亚基因组成分渐渗的“新型”甘蓝型油菜，拓宽了“传统”甘蓝型油菜的遗传基础。本研究以不同国家的甘蓝型油菜新品种和传统甘蓝型油菜品种作为两个不同的群体，通过与前人连锁作图研究的比较，评价了甘蓝型油菜种子含油量，并确定了关联作图的效果。新型甘蓝型油菜遗传多样性相对丰富，但与传统甘蓝型油菜相比，连锁不平衡程度较高。同样，在新型甘蓝型油菜群体中，检测到更大的含油量变异和更多的相关标记。与此同时，相关标记所对应的半数以上的遗传位点位于先前通过连锁作图实验确定的数量性状位点区间内，证明了甘蓝型油菜关联作图的威力。
Association mapping has been used increasingly in natural populations with rich genetic diversity to detect DNA-based markers that are associated with important agronomic traits. Brassica napus is an important oil crop with limited genetic diversity. "New-type" B. napus that is introgressed with subgenomic components from related species has been developed to broaden the genetic basis of "traditional" B. napus. In this study, new-type B. napus lines and a collection of traditional B. napus varieties from different countries were used as two different populations to evaluate seed oil content and to determine the efficacy of association mapping by comparison with previous study of linkage mapping. Relatively rich genetic diversity, but a higher level of linkage disequilibrium was observed in the new-type B. napus as compared with the traditional B. napus. Similarly, a larger variation in oil content and a greater number of associated markers were detected in the population of new-type B. napus. Meanwhile, more than half of the genetic loci, to which the associated markers corresponded, were located within the quantitative trait loci intervals identified previously in linkage mapping experiments, which demonstrated the power of association mapping in B. napus.