Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen
Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen
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DOI：
10.1038/35023079
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发表时间：
2000-08-31
期刊：
影响因子：
64.8
通讯作者：
Olson, MV
中科院分区：
文献类型：
--
作者：
Stover, CK;Pham, XQ;Olson, MV
铜绿假单胞菌是一种普遍存在的环境细菌，是人类机会性感染的三大原因之一。它作为病原体的突出地位的一个主要因素是它对抗生素和消毒剂的内在耐药性。在这里，我们报告了铜绿假单胞菌菌株PAO1的完整序列。在630万个碱基对，这是最大的细菌基因组测序，该序列提供了对铜绿假单胞菌的多功能性和内在耐药性的基础的见解。与其较大的基因组大小和环境适应性相一致，铜绿假单胞菌包含细菌基因组中观察到的最高比例的调控基因和大量参与有机化合物的催化、运输和外排的基因以及四种潜在的趋化系统。我们认为，铜绿假单胞菌基因组的大小和复杂性反映了一种进化适应，使其能够在不同的环境中茁壮成长，并抵抗各种抗菌物质的影响。
Pseudomonas aeruginosa is a ubiquitous environmental bacterium that is one of the top three causes of opportunistic human infections. A major factor in its prominence as a pathogen is its intrinsic resistance to antibiotics and disinfectants. Here we report the complete sequence of P. aeruginosa strain PAO1. At 6.3 million base pairs, this is the largest bacterial genome sequenced, and the sequence provides insights into the basis of the versatility and intrinsic drug resistance of P. aeruginosa. Consistent with its larger genome size and environmental adaptability, P. aeruginosa contains the highest proportion of regulatory genes observed for a bacterial genome and a large number of genes involved in the catabolism, transport and efflux of organic compounds as well as four potential chemotaxis systems. We propose that the size and complexity of the P. aeruginosa genome reflect an evolutionary adaptation permitting it to thrive in diverse environments and resist the effects of a variety of antimicrobial substances.