Histochemical and cytological characterizations of mucosal and connective tissue mast cells of Mongolian gerbils (Meriones unguiculatus).
Histochemical and cytological characterizations of mucosal and connective tissue mast cells of Mongolian gerbils (Meriones unguiculatus).
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DOI：
10.1159/000236673
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发表时间：
1994-07
影响因子：
2.8
通讯作者：
Y. Nawa;Y. Horii;M. Okada;N. Arizono
中科院分区：
文献类型：
--
作者：
Y. Nawa;Y. Horii;M. Okada;N. Arizono
本文对长爪沙鼠空肠、皮肤和舌中的肥大细胞进行了组织化学、酶组织化学和超微结构的研究。当通过测量临界电解质浓度来表征空肠和舌中肥大细胞的糖胺聚糖时，空肠和舌中50%肥大细胞可染色的盐浓度> 1.0 M，表明两个部位的肥大细胞均含有肝素。酶组织化学研究表明，蒙古沙鼠空肠肥大细胞糜蛋白酶和类胰蛋白酶呈强阳性，而舌和皮肤的肥大细胞基本上为阴性。电镜观察发现，空肠肥大细胞的颗粒形态和表面微褶的分布与耳皮肤肥大细胞的颗粒形态和表面微褶的分布不同。然而，在用化合物48/80体内刺激后，两个部位的肥大细胞均显示出显著的脱粒。从这些结果来看，虽然蒙古沙鼠也有不同的肥大细胞亚群，但它们的表型特性与先前已知的其他动物的肥大细胞亚群不同。
Mast cells in jejunum, skin, and tongue of Mongolian gerbils (Meriones unguiculatus) were examined in terms of their histochemical, enzyme-histochemical, and ultrastructural properties. When glycosaminoglycans of mast cells in jejunum and tongue were characterized by measuring the critical electrolyte concentration, the salt concentration at which 50% of mast cells could be stained was > 1.0 M for those in the jejunum and also in the tongue, indicating that mast cells in both sites contained heparin. Enzyme-histochemical study revealed that mast cells in the jejunum of Mongolian gerbils were strongly positive for chymase and tryptase, whereas those in tongue and skin were essentially negative for both proteases. By electron microscopy, granular morphology and distribution of surface microfolds of mast cells in the jejunum were different from those in the ear skin. After in vivo stimulation with compound 48/80, however, mast cells in both sites showed remarkable degranulation. From these results, although Mongolian gerbils also have distinct mast cell subsets, their phenotypic properties are different from those of the previously known mast cell subsets of other animals.