Photoionization of Oxidized Coenzyme Q in Microemulsion: Laser Flash Photolysis Study in Biomembrane‐like System
Photoionization of Oxidized Coenzyme Q in Microemulsion: Laser Flash Photolysis Study in Biomembrane‐like System
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微乳液中氧化辅酶 Q 的光电离：类生物膜系统中的激光闪光光解研究
DOI：
10.1111/j.1751-1097.2012.01180.x
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发表时间：
2013
影响因子：
3.3
通讯作者：
Shi
中科院分区：
文献类型：
--
作者：
Kun Li;Mei Wang;Jin Wang;Rongrong Zhu;Dongmei Sun;Xiao;Shi
在均相体系中，光激发产生三重态是包括氧化辅酶Q(CoQ)及其类似物在内的许多苯醌衍生物的主要光反应。在本研究中，辅酶Q微乳液是一种非均相体系，用来模拟辅酶Q在生物膜中的分布。利用激光闪光光解技术对CoQ10在微乳液和环己烷中的光化学进行了研究和比较，结果表明，CoQ10在微乳液和环己烷中发生单光子光离，生成CoQ10的自由基阳离子，在环己烷中光激发生成激发三重态。同时，还考察了DQ和CoQ0在微乳液中的光反应，分析了分子结构对微乳液中苯醌衍生物光化学性质的影响。结果表明，在微乳液中，DQ和CoQ0的主要光反应是光激发，其次是激发态的吸氢反应。然而，光激发的CoQ0也会导致水合电子的形成。提出了类异戊二烯侧链参与的高共振稳定性解释了CoQ0和CoQ10在微乳液中光反应的差异。考虑到微乳与生物膜体系关系密切，其在微乳液中的光致电离可能有助于理解生物膜体系中生物对苯二酚的真实光化学。
Photoexcitation to generate triplet state has been proved to be the main photoreaction in homogeneous system for many benzoquinone derivatives, including oxidized coenzyme Q (CoQ) and its analogs. In the present study, microemulsion of CoQ, a heterogeneous system, is employed to mimic the distribution of CoQ in biomembrane. The photochemistry of CoQ10 in microemulsion and cyclohexane is investigated and compared using laser flash photolysis and results show that CoQ10 undergoes photoionization via a monophotonic process to generate radical cation of CoQ10 in microemulsion and photoexcitation to generate excited triplet state in cyclohexane. Meanwhile, photoreactions of duroquinone (DQ) and CoQ0 in microemulsion are also investigated to analyze the influence of molecular structure on the photochemistry of benzoquinone derivatives in microemulsion. Results suggest that photoexcitation, which is followed by excited state‐involved hydrogen‐abstraction reaction, is the main photoreaction for DQ and CoQ0 in microemulsion. However, photoexcited CoQ0 also leads to the formation of hydrated electrons. The isoprenoid side chain‐involved high resonance stabilization is proposed to explain the difference in photoreactions of CoQ0 and CoQ10 in microemulsion. Considering that microemulsion is close to biomembrane system, its photoionization in microemulsion may be helpful to understand the real photochemistry of biological quinones in biomembrane system.
DOI：
10.1016/0006-291x(90)91971-t
发表时间：
1990-06-29
影响因子：
3.1
作者：
KAGAN, V;SERBINOVA, E;PACKER, L
通讯作者：
PACKER, L
不同链长的泛醌在人工膜囊泡中的位置和迁移率。
影响因子：
2.9
作者：
Ulrich,EL;Girvin,ME;Cramer,WA;Markley,JL
通讯作者：
Markley,JL
影响因子：
7.4
作者：
KAGAN, VE;SERBINOVA, EA;PACKER, L
通讯作者：
PACKER, L