SUBCELLULAR COMPARTMENTATION IN CONTROL OF CONVERGING PATHWAYS FOR PROLINE AND ARGININE METABOLISM IN SACCHAROMYCES-CEREVISIAE
SUBCELLULAR COMPARTMENTATION IN CONTROL OF CONVERGING PATHWAYS FOR PROLINE AND ARGININE METABOLISM IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1128/jb.145.3.1359-1364.1981
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发表时间：
1981-01-01
影响因子：
3.2
通讯作者：
MAGASANIK, B
中科院分区：
文献类型：
--
作者：
BRANDRISS, MC;MAGASANIK, B
作用于同一化合物DELTA1-吡咯烷-5-羧酸盐的脯氨酸生物合成和降解酶在酵母细胞中物理上是分开的。负责脯氨酸生物合成的最后一步的酶，吡咯烷-5-羧酸还原酶，将吡咯烷-5-羧酸转化为脯氨酸，位于细胞质中。脯氨酸降解途径中的最后一种酶，吡咯烷-5-羧酸脱氢酶，将吡咯烷-5-羧酸转化为谷氨酸，存在于细胞的颗粒部分，可能存在于线粒体中。通过亚细胞分隔，酵母细胞避免了在脯氨酸和吡咯烷-5-羧酸之间的无用循环。
Enzymes of proline biosynthesis and proline degradation which act on the same compound, .DELTA.1-pyrroline-5-carboxylate, are physically separated in yeast cells. The enzyme responsible for the final step in proline biosynthesis, pyrroline-5-carboxylate reductase, converts pyrroline-5-carboxylate to proline and is located in the cytoplasm. The last enzyme in the proline degradative pathway, pyrroline-5-carboxylate dehydrogenase, converts pyrroline-5-carboxylate to glutamate and is found in the particulate fraction of the cell, presumably in the mitochondrion. By subcellular compartmentation, yeast cells avoid futile cycling between proline and pyrroline-5-carboxylate.