LEUKOCYTE ROLLING AND EXTRAVASATION ARE SEVERELY COMPROMISED IN P-SELECTIN-DEFICIENT MICE
LEUKOCYTE ROLLING AND EXTRAVASATION ARE SEVERELY COMPROMISED IN P-SELECTIN-DEFICIENT MICE
复制标题
DOI：
10.1016/0092-8674(93)80055-j
复制
发表时间：
1993-08-13
期刊：
影响因子：
64.5
通讯作者：
WAGNER, DD
中科院分区：
文献类型：
--
作者：
MAYADAS, TN;JOHNSON, RC;WAGNER, DD
P选择素是白细胞的粘附受体，表达于活化的内皮细胞和血小板表面。我们报告说，P选择素缺陷的小鼠，产生的基因靶向胚胎干细胞，表现出一些缺陷的白细胞行为，包括循环中性粒细胞的数量增加，几乎完全没有白细胞滚动在肠系膜小静脉，和延迟招聘的中性粒细胞到腹腔实验诱导的炎症。这些结果清楚地证明了P选择素在白细胞与血管壁的相互作用以及炎症部位白细胞募集的早期步骤中的作用。这些突变小鼠应证明在破译的各种炎症反应以及血小板功能的P选择素的贡献是有用的。
P selectin, expressed on surfaces of activated endothelial cells and platelets, is an adhesion receptor for leukocytes. We report that P selectin-deficient mice, generated by gene targeting in embryonic stem cells, exhibit a number of defects in leukocyte behavior, including elevated numbers of circulating neutrophils, virtually total absence of leukocyte rolling in mesenteric venules, and delayed recruitment of neutrophils to the peritoneal cavity upon experimentally induced inflammation. These results clearly demonstrate a role for P selectin in leukocyte interactions with the vessel wall and in the early steps of leukocyte recruitment at sites of inflammation. These mutant mice should prove useful in deciphering the contributions of P selectin in various inflammatory responses as well as in platelet functions.