In Vitro and In Vivo Human Herpesvirus 8 Infection of Placenta
In Vitro and In Vivo Human Herpesvirus 8 Infection of Placenta
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DOI：
10.1371/journal.pone.0004073
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发表时间：
2008-12-30
期刊：
影响因子：
3.7
通讯作者：
Fiore, Jose Ramon
中科院分区：
文献类型：
--
作者：
Di Stefano, Mariantonietta;Calabro, Maria Luisa;Fiore, Jose Ramon
胎盘疱疹病毒感染可能对妊娠有害，导致胎儿生长障碍、早产、流产或严重先天性畸形。虽然人类疱疹病毒8型（HHV-8）感染与流产或低出生体重的儿童之间的相关性已被提出，和罕见的情况下，在子宫内或围产期HHV-8传播已被记录，没有直接的证据HHV-8感染的胎盘尚未报告。本研究的目的是评估胎盘细胞对HHV-8感染的体外和体内易感性。对从足月胎盘分离的胎盘绒毛膜绒毛进行短期感染试验。采用PCR和实时荧光定量PCR方法对HHV-8进行定性和定量检测，并采用免疫组化方法对HHV-8蛋白进行分析。对来自HHV-8血清阳性妇女的足月胎盘样本进行HHV-8 DNA和抗原的分析。在体外感染的组织培养显示组织和上清液中HHV-8 DNA的量增加;细胞和合胞体滋养层，以及内皮细胞，表达潜伏和裂解病毒抗原。在感染的组织培养物中，通过末端脱氧核苷酸转移酶介导的脱氧尿苷缺口末端标记法观察到凋亡现象增加。体外，HHV-8 DNA和潜伏病毒抗原检测胎盘样本HHV-8血清阳性的妇女。这些发现表明，HHV-8，像其他人类疱疹病毒，可以感染胎盘细胞在体外和体内，从而提供证据表明，这种现象可能会影响垂直传播和妊娠结局的HHV-8感染的妇女。
Herpesvirus infection of placenta may be harmful in pregnancy leading to disorders in fetal growth, premature delivery, miscarriage, or major congenital abnormalities. Although a correlation between human herpesvirus 8 (HHV-8) infection and abortion or low birth weight in children has been suggested, and rare cases of in utero or perinatal HHV-8 transmission have been documented, no direct evidence of HHV-8 infection of placenta has yet been reported. The aim of this study was to evaluate the in vitro and in vivo susceptibility of placental cells to HHV-8 infection. Short-term infection assays were performed on placental chorionic villi isolated from term placentae. Qualitative and quantitative HHV-8 detection were performed by PCR and real-time PCR, and HHV-8 proteins were analyzed by immunohistochemistry. Term placenta samples from HHV-8-seropositive women were analyzed for the presence of HHV-8 DNA and antigens. In vitro infected histocultures showed increasing amounts of HHV-8 DNA in tissues and supernatants; cyto- and syncitiotrophoblasts, as well as endothelial cells, expressed latent and lytic viral antigens. Increased apoptotic phenomena were visualized by the terminal deoxynucleotidyl transferase-mediated deoxyuridine nick end-labeling method in infected histocultures. Ex vivo, HHV-8 DNA and a latent viral antigen were detected in placenta samples from HHV-8-seropositive women. These findings demonstrate that HHV-8, like other human herpesviruses, may infect placental cells in vitro and in vivo, thus providing evidence that this phenomenon might influence vertical transmission and pregnancy outcome in HHV-8-infected women.