Structural basis for mRNA and tRNA positioning on the ribosome
Structural basis for mRNA and tRNA positioning on the ribosome
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DOI：
10.1073/pnas.0607541103
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发表时间：
2006-10-24
影响因子：
11.1
通讯作者：
Cate, Jamie H. Doudna
中科院分区：
文献类型：
--
作者：
Berk, Veysel;Zhang, Wen;Cate, Jamie H. Doudna
蛋白质合成需要信使核糖核酸（mRNA）和转运核糖核酸（tRNA）在核糖体的肽酰 - tRNA位点准确定位。在此我们描述了来自大肠杆菌的完整细菌核糖体与mRNA以及P位点tRNA的反密码子茎环形成复合物的X射线晶体结构。在3.5埃分辨率下，这些结构揭示了完整核糖体中的重排，这种重排将P位点tRNA和mRNA固定在核糖体小亚基上。P位点tRNA的反密码子茎环与核糖体的结合足以将核糖体小亚基的头部锁定在单一构象，从而在mRNA解码前阻止mRNA和tRNA的移动。
Protein synthesis requires the accurate positioning of mRNA and tRNA in the peptidyl-tRNA site of the ribosome. Here we describe x-ray crystal structures of the intact bacterial ribosome from Escherichia coli in a complex with mRNA and the anticodon stem-loop of P-site tRNA. At 3.5-angstrom resolution, these structures reveal rearrangements in the intact ribosome that clamp P-site tRNA and mRNA on the small ribosomal subunit. Binding of the anticodon stem-loop of P-site tRNA to the ribosome is sufficient to lock the head of the small ribosomal subunit in a single conformation, thereby preventing movement of mRNA and tRNA before mRNA decoding.