Clipping of Telomere from Human Chromosomes using Chemistry-based Artificial Restriction DNA Cutter
Clipping of Telomere from Human Chromosomes using Chemistry-based Artificial Restriction DNA Cutter
复制标题
使用基于化学的人工限制性 DNA 切割器从人类染色体上剪下端粒
DOI：
10.1002/0471142700.nc0613s61
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Makoto Komiyama
中科院分区：
文献类型：
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作者：
Takumi Ishizuka;Yan Xu;Makoto Komiyama
检测人类染色体的单个端粒长度可以为基因组稳定性、癌症和端粒相关疾病提供重要信息。然而，目前测量端粒长度的方法有缺点，限制了它们的使用。最近，我们开发了一种检测个体端粒长度（DITL）的方法，该方法使用基于化学的DNA切割方法。DITL方法最有益的特点是切割端粒附近的序列，然后在单个染色体的核苷酸水平上分辨端粒长度。在本单元中，详细描述了从单个染色体成功检测单个端粒长度的协议。©2015 by John Wiley & Sons, Inc。
The detection of individual telomere lengths of human chromosomes can provide crucial information on genome stability, cancer, and telomere‐related diseases. However, current methods to measure telomere length entail shortcomings that have limited their use. Recently, we have developed a method for detection of individual telomere lengths (DITL) that uses a chemistry‐based DNA‐cutting approach. The most beneficial feature of the DITL approach is to cleave the sequence adjacent to the telomere followed by resolution of the telomere length at the nucleotide level of a single chromosome. In this unit, a protocol for successful detection of individual telomere lengths from individual chromosomes is described in detail. © 2015 by John Wiley & Sons, Inc.