Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors
Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors
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DOI：
10.1038/nmeth.1207
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发表时间：
2008-05-01
期刊：
影响因子：
48
通讯作者：
Campbell, Robert E.
中科院分区：
文献类型：
--
作者：
Ai, Hui-wang;Hazelwood, Kristin L.;Campbell, Robert E.
荧光蛋白质的荧光共振能量转移(FRET)是一种检测细胞内蛋白质相互作用、酶活性和小分子的有效方法。借助于一种新的紫色可激发的黄色荧光突变体Aequorea Victoria GFP，我们开发了基于双重FRET的caspase-3生物传感器。由于它们不同的激发曲线，每个FRET生物传感器都可以在另一个存在的情况下按比例成像。
Fluorescence resonance energy transfer (FRET) with fluorescent proteins is a powerful method for detection of protein-protein interactions, enzyme activities and small molecules in the intracellular milieu. Aided by a new violet-excitable yellow-fluorescing variant of Aequorea victoria GFP, we developed dual FRET-based caspase-3 biosensors. Owing to their distinct excitation profiles, each FRET biosensor can be ratiometrically imaged in the presence of the other.