DEVELOPMENT AND FUNCTIONS OF THE CYTOSKELETON DURING CILIOGENESIS IN METAZOA
DEVELOPMENT AND FUNCTIONS OF THE CYTOSKELETON DURING CILIOGENESIS IN METAZOA
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DOI：
10.1016/0248-4900(88)90058-5
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发表时间：
1988-01-01
影响因子：
2.7
通讯作者：
SANDOZ, D
中科院分区：
文献类型：
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作者：
LEMULLOIS, M;BOISVIEUXULRICH, E;SANDOZ, D
鹌鹑输卵管中纤毛发生的不同步骤与其他后生动物中描述的纤毛发生模式进行了比较。中心粒是根据在同一细胞中发现的途径产生的：中心粒和无中心粒途径。在无中心粒途径中，中心粒在高尔基体区域产生，不与中心体的预先存在的中心粒接触，并且它们向顶膜迁移。通过几种药物（秋水仙碱、诺考达唑、紫杉醇、细胞松弛素D、苯二氮卓类）和免疫细胞化学研究了这种极化迁移的控制。结果表明，肌动蛋白-肌球蛋白系统参与了中心粒的迁移，而不稳定的微管是不必要的。基体必须与质膜或细胞质囊泡对接，以启动轴丝微管聚合。即使在紫杉醇存在下，这种信号也是必需的。
The different steps of ciliogenesis occurring in quail oviduct were compared to the ciliogenesis pattern described in other metazoan species.Centrioles are generated according to pathways that are found within the same cell: the centriolar and the acentriolar pathways. In the acentriolar pathway, centrioles are generated in the Golgi area, without contact with the preexisting centrioles of the centrosomes, and they migrate toward the apical membrane. The control of this polarized migration was studied by means of several drugs (colchicine, nocodazol, taxol, cytochalasin D, benzodiazepines) and immunocytochemistry.It was suggested that an actin‐myosin system was involved in the migration of centrioles, whereas labile microtubules were not necessary.Basal bodies must dock with plasma membrane or cytoplasmic vesicles for the initiation of axonemal microtubule polymerization. This signal is necessary even in the presence of taxol.