Holographic aberration correction: optimising the stiffness of an optical trap deep in the sample.
Holographic aberration correction: optimising the stiffness of an optical trap deep in the sample.
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DOI：
10.1364/oe.19.024589
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发表时间：
2011-11
期刊：
影响因子：
3.8
通讯作者：
M. Dienerowitz;G. Gibson;R. Bowman;M. Padgett
中科院分区：
文献类型：
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作者：
M. Dienerowitz;G. Gibson;R. Bowman;M. Padgett
研究了1（st）阶球差和离焦对进入样品数十μm的光阱刚度的影响。我们用空间光调制器来控制这两种像差。在一定深度范围内保持最佳阱刚度的关键是散焦和轴向物镜位置的特定非平凡组合。这种优化将陷阱刚度提高了3倍，并允许在样品中捕获深度为50 μm的1 μm聚苯乙烯珠。
We investigate the effects of 1(st) order spherical aberration and defocus upon the stiffness of an optical trap tens of μm into the sample. We control both these aberrations with a spatial light modulator. The key to maintain optimum trap stiffness over a range of depths is a specific non-trivial combination of defocus and axial objective position. This optimisation increases the trap stiffness by up to a factor of 3 and allows trapping of 1 μm polystyrene beads up to 50 μm deep in the sample.