Iron deposition and ferritin heavy chain (Fth) localization in rodent teeth.
Iron deposition and ferritin heavy chain (Fth) localization in rodent teeth.
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DOI：
10.1186/1756-0500-6-1
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发表时间：
2013-01-02
影响因子：
1.8
通讯作者：
Paine ML
中科院分区：
文献类型：
--
作者：
Wen X;Paine ML
在唇面上的富铁层是啮齿动物门牙的釉质的特征。为了解决铁含量的作用，在不断增长的切牙在牙发育过程中，我们研究了铁沉积模式，在釉质和牙本质使用Perls蓝染色和铁蛋白重链（Fth）免疫定位。Fth的表达受铁水平的调节，因此其定位可作为铁沉积的敏感指标。4周龄大鼠切牙的釉质切片显示，从分泌期到成熟期，成釉器中的铁水平逐渐增加。此外，铁在4周龄大鼠萌出的第三磨牙的成釉细胞中检测到，表明铁在切牙和磨牙发育中起作用。在成牙本质细胞中，铁的存在被证明，这与铁在胶原蛋白合成中的作用是一致的。使用出生后3-，6-，9天的小鼠，在牙齿发育的Fth的时空表达再次表明，在成熟的成釉细胞和成牙本质细胞中存在铁。虽然这些数据不能解释铁在牙齿形成中的功能作用，但它确实突出了与啮齿动物牙列形成相关的重要分子活性。
An iron rich layer on the labial surface is characteristic of the enamel of rodent incisors. In order to address a role for iron content in continuously growing incisors during odontogenesis, we studied iron deposition patterns in enamel and dentine using Perls’ blue staining and ferritin heavy chain (Fth) immunolocalization. Fth expression is regulated by iron level; therefore its localization can be used as a sensitive indicator for iron deposition. Sagittal sections of 4-week old rat incisors showed a gradual increase in iron level in the enamel organ from secretory to maturation stages. In addition, iron was detected in ameloblasts of erupting third molars of 4-week old rats, suggesting iron plays a role in both incisor and molar development. In odontoblasts, the presence of iron was demonstrated, and this is consistent with iron’s role in collagen synthesis. Using postnatal 3-, 6-, 9-day old mice, the spatial and temporal expression of Fth in tooth development again indicated the presence of iron in mature ameloblasts and odontoblasts. While these data do not explain what functional role iron has in tooth formation, it does highlight a significant molecular activity associated with the formation of the rodent dentition.