Localization of nerve growth factor (NGF) receptors in the mitochondrial compartment: Characterization and putative role
Localization of nerve growth factor (NGF) receptors in the mitochondrial compartment: Characterization and putative role
复制标题
DOI：
10.1016/j.bbagen.2011.10.015
复制
发表时间：
2012-02-01
影响因子：
3
通讯作者：
Caroleo, Maria Cristina
中科院分区：
文献类型：
--
作者：
Carito, Valentina;Pingitore, Attilio;Caroleo, Maria Cristina
背景资料：神经营养因子NGF受体trkA和p75 NTR在中枢和外周神经系统以及非神经元组织中表达;最初被描述为定位于质膜，最近的研究表明这两种NGF受体的其他细胞内定位。为了确定NGF受体是否定位于从人肾脏分离的线粒体区室线粒体，大鼠组织和人类足细胞作为细胞系分化前后used.Major结论：我们的研究结果表明，神经生长因子受体定位于未分化的人类足细胞的线粒体室，并在所有组织分析，包括大鼠中枢神经系统。在线粒体中，p75 NTR，而不是trkA，与腺嘌呤核苷酸转运蛋白（ANT）和磷酸二酯酶4亚型A5（PDE 4A 5）共免疫沉淀。此外，神经生长因子，通过trkA，保护大鼠脑皮层线粒体的线粒体渗透性转换诱导的Ca 2+。一般意义：虽然神经生长因子受体已被描述为主要citoplasmic到目前为止，我们证明了他们的表达在线粒体水平和他们的相互作用与特定的蛋白质的证据。我们的研究结果表明，在线粒体中的神经生长因子受体的表达提供了新的见解神经生长因子在亚细胞水平上的作用，在不同领域的有机体，包括中枢神经系统。(C)2011爱思唯尔有限公司版权所有。
Background: The neurotrophin NGF receptors trkA and p75NTR are expressed in the central and peripheral nervous system as well as in non-neuronal tissues; originally described to localize to the plasma membrane, recent studies have suggested other intracellular localizations for both NGF receptors.Scope of review: In order to determine whether NGF receptors localize to the mitochondrial compartment mitochondria isolated from human kidney, rat tissues and a human podocyte as cell line before and after differentiation were used.Major conclusions: Our results demonstrate that NGF receptors are localized in the mitochondrial compartment of undifferentiated human podocytes and in all tissues analyzed including rat central nervous system. In mitochondria p75NTR, but not trkA, co-immunoprecipitates with the adenine nucleotide translocator (ANT) and the phosphodiesterase 4 isoform A5 (PDE4A5). Moreover, NGF, via trkA, protects isolated mitochondria of rat brain cortex from mitochondrial permeability transition induced by Ca2+.General significance: Although NGF receptors have been described as mainly citoplasmatic so far, we proved evidence of their expression at the mitochondrial level and their interaction with specific proteins. Our results demonstrating the expression of NGF receptors in the mitochondria provide new insights into the role of NGF at subcellular level, in different areas of the organism, including CNS. (C) 2011 Elsevier B.V. All rights reserved.