Fluorescence detection of protamine, heparin and heparinase II based on a novel AIE molecule with four carboxyl
Fluorescence detection of protamine, heparin and heparinase II based on a novel AIE molecule with four carboxyl
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基于新型四羧基AIE分子的鱼精蛋白、肝素和肝素酶II的荧光检测
DOI：
10.1016/j.ijbiomac.2019.11.150
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发表时间：
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影响因子：
8.2
通讯作者：
Yan Zhang
中科院分区：
文献类型：
--
作者：
Rui Jiang;Song Zhao;Langkun Chen;Maoyu Zhao;Wenjing Qi;Wensheng Fu;Lianzhe Hu;Yan Zhang
本研究设计并合成了一种具有四个羧基的新型DSA（二苯乙烯基蒽）衍生物。该分子表现出聚集诱导发射特性（AIE）。利用AIE的特性，开发了一种方便、灵敏的荧光探针，用于检测鱼精蛋白、肝素和肝素酶。鱼精蛋白可以直接引起探针的聚集，这是由静电吸引引起的。这样就实现了鱼精蛋白的开启检测，检测限低至30ngmL−1。当肝素出现时，探针会重新分散在溶液中，导致荧光强度降低。此外，该方法还表现出良好的选择性和灵敏度，肝素的线性范围为0.08至8 μg mL−1，检出限为37 ng mL−1。肝素酶水解肝素后，探针与鱼精蛋白重新结合，荧光增强。在 0.02–2.0 µg mL−1 范围内，荧光增强与肝素酶浓度呈线性相关，检测限低至 143.7 ng mL−1。此外，结果显示牛样品中肝素酶的回收率达到96%~101%。
In this research, a new DSA (Distyryl-anthracene) derivative with four carboxyl groups was designed and synthesized. This molecule exhibits aggregation-induced emission property (AIE). With the AIE character, a convenient and sensitive fluorescent probe for the detection of protamine, heparin and heparinase has been developed. The protamine can directly induce the aggregation of probe, which caused by electrostatic attraction. In this way, the turn-on detection of protamine is achieved, and the detection limit is as low as 30 ng mL−1. When heparin appears, the probes will be redisperse in solution, which causes a decrease in fluorescence intensity. Besides, this method also shows good selectivity and sensitivity, and the linear range of heparin is from 0.08 to 8 μg mL−1with detection limit of 37 ng mL−1. After hydrolyzing heparin by heparinase, the probes rebind with protamine and the fluorescence enhance. The fluorescence enhancement was linearly related to the concentration of heparinase in the range of 0.02–2.0 μg mL−1and detection limit as low as 143.7 ng mL−1. In addition, the results exhibited that the recovery percentage of heparinase in bovine samples reached to 96–101%.